| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ramos, Daniela I (9)
|
NIUNAC |
59.33 |
50.34 |
-8.99 |
| 2 |
Boyer, Chloe G (9)
|
NIISWM |
1:01.03 |
51.85 |
-9.18 |
| 3 |
Melveney, Emily R (10)
|
NIGGST |
57.00 |
57.06 |
0.06 |
| 4 |
Denlinger, Elizabeth J (10)
|
NIWAAC |
|
57.18 |
- |
| 5 |
Blakely-Armitage, Fiona S (9)
|
NIISWM |
53.86 |
57.20 |
3.34 |
| 6 |
Vanderhoof, Emma J (10)
|
NIWAAC |
|
1:07.64 |
- |
|
Roberts, Olivia M (9)
|
NIHAWK |
1:13.47 |
DQ |
- |
|
Williams, Lauren K (9)
|
NIHAWK |
1:25.40 |
DQ |
- |
|
Leahey, Emme A (9)
|
NIUNAC |
|
DQ |
- |
|
Ryan, Natalie C (9)
|
NIUNAC |
1:13.84 |
DQ |
- |
|
Leonard, Kaitllyn S (9)
|
NIWAAC |
|
DQ |
- |