| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
LU, Jinjin (13)
|
ORTEAM |
31.40 |
33.72 |
2.32 |
| 2 |
Borges, Emily S (13)
|
ORRYST |
33.99 |
34.42 |
0.43 |
| 3 |
Brenden, Nora (13)
|
ORMPY |
|
35.76 |
- |
| 4 |
Todd, Amber R (13)
|
ORRYST |
39.09 |
37.47 |
-1.62 |
| 5 |
Thornton, Katherine E (13)
|
ORTOR |
38.72 |
37.80 |
-0.92 |
| 6 |
Cooper, Lilianna J (13)
|
ORTOR |
40.79 |
37.85 |
-2.94 |
| 7 |
Blue, Kathryn E (14)
|
ORRYST |
45.50 |
38.19 |
-7.31 |
| 8 |
Howard, Alena K (13)
|
ORRYST |
40.24 |
38.81 |
-1.43 |
| 9 |
Christie, Mia R (13)
|
ORSYD |
39.80 |
41.28 |
1.48 |
| 10 |
Jett, Molly J (13)
|
ORTEAM |
44.90 |
45.20 |
0.30 |
| 11 |
Brintnall, Camille K (13)
|
ORTEAM |
|
47.95 |
- |
|
Ayotte, Tayler J (13)
|
ORSYD |
|
DQ |
- |
|
McNamara, Tilly M (13)
|
ORTEAM |
47.01 |
NS |
- |