| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mottel, Lauren G (14)
|
MADST |
2:28.50 |
2:33.40 |
4.90 |
| 1 |
Mummert, Lauren E (14)
|
MACYA |
2:57.05 |
2:56.71 |
-0.34 |
| 1 |
Wagner, Kelsey D (13)
|
MABRY |
|
3:17.46 |
- |
| 2 |
Pfaff, Rabea K (14)
|
MADST |
2:35.85 |
2:41.28 |
5.43 |
| 2 |
Reedy, Kayla M (13)
|
MACYA |
|
2:57.97 |
- |
| 2 |
Singh, Srishti (14)
|
MACYA |
|
3:45.14 |
- |
| 3 |
Boyes, Samantha R (13)
|
MATHY |
2:57.54 |
2:44.96 |
-12.58 |
| 3 |
Kesselring, Tatiana N (14)
|
MDFSC |
|
2:59.79 |
- |
| 4 |
Liu, Chelsey (13)
|
MADST |
2:37.44 |
2:45.73 |
8.29 |
| 4 |
Monaghan, Abaigeal Ann P (13)
|
MATHY |
3:06.78 |
2:59.89 |
-6.89 |
| 5 |
Eyler, Madisen H (14)
|
MDFSC |
2:58.97 |
3:01.22 |
2.25 |