| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shaffer, Sabrina A (13)
|
MAPCAC |
2:34.98 |
2:34.17 |
-0.81 |
| 2 |
Lopez-Ona, Gabriella A (13)
|
MAPCAC |
2:31.23 |
2:36.15 |
4.92 |
| 3 |
Thamm, Claudia M (13)
|
MANPAC |
2:37.28 |
2:39.01 |
1.73 |
| 4 |
Mikhalev, Vitalina (14)
|
MACBST |
|
2:48.78 |
- |
| 5 |
Gally, Annika L (13)
|
MABYNS |
2:55.70 |
2:55.51 |
-0.19 |
| 6 |
Volpp, Anna (13)
|
MAFCA |
3:02.71 |
3:02.64 |
-0.07 |
| 7 |
Gerstenberg, Alyssa L (14)
|
MAKIDS |
|
3:07.72 |
- |
| 8 |
Novacich, Isabel (13)
|
MAFCA |
3:16.07 |
3:16.22 |
0.15 |
| 9 |
Alulis, Rachael A (14)
|
MAKIDS |
3:17.71 |
3:18.90 |
1.19 |
| 10 |
Nagle, Molly E (14)
|
MAKIDS |
3:13.92 |
3:26.41 |
12.49 |