| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Redman, Emma L (16)
|
PVFISH |
33.64 |
37.01 |
3.37 |
| 2 |
Pfisterer, Brooke E (16)
|
MRSSC |
38.20 |
38.30 |
0.10 |
| 3 |
Watts, Emma C (15)
|
PVFISH |
36.19 |
38.41 |
2.22 |
| 4 |
Mulkey, Anne M (15)
|
PVNCAP |
41.94 |
38.84 |
-3.10 |
| 5 |
Do, Clara T (17)
|
PVFISH |
39.29 |
41.81 |
2.52 |
| 6 |
Pike, Olivia J (16)
|
PVNCAP |
42.00 |
44.08 |
2.08 |
| 7 |
Mackay, Victoria A (16)
|
MRSSC |
43.89 |
44.23 |
0.34 |
| 8 |
Calandra, Lily A (15)
|
MRSSC |
44.47 |
45.93 |
1.46 |
| 9 |
Vanhorn, Charlotte B (16)
|
PVTOLL |
45.50 |
46.04 |
0.54 |
| 10 |
Mackay, Alexandria E (17)
|
MRSSC |
45.08 |
47.95 |
2.87 |
| 11 |
Mahady, Madison G (15)
|
MRSSC |
48.20 |
48.26 |
0.06 |
| 12 |
McPhail, Madison R (15)
|
MDHFY |
50.81 |
48.87 |
-1.94 |
| 13 |
Cialdella, Marina L (17)
|
NJBWTD |
49.25 |
49.78 |
0.53 |
| 14 |
Glennon, Abigail K (17)
|
MRSSC |
48.24 |
49.87 |
1.63 |
| 15 |
Windle, Blythe M (16)
|
PVTOLL |
51.00 |
57.63 |
6.63 |
|
Vertichio, Kaylie A (18)
|
MRSSC |
42.69 |
DQ |
- |
|
Carvallo, Valeria V (18)
|
PVTANK |
50.00 |
NS |
- |
|
Medina, Claudia A (15)
|
PVTANK |
51.00 |
NS |
- |
|
Aguilera, Daysi D (16)
|
PVTANK |
52.72 |
NS |
- |
|
Burke, Paige F (16)
|
PVNCAP |
39.86 |
NS |
- |