| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fisk, Holly E (13)
|
NIFAST |
36.27 |
35.72 |
-0.55 |
| 2 |
Carter, Owen T (11)
|
NIFAST |
36.48 |
36.77 |
0.29 |
| 3 |
Webb, Karen R (11)
|
NIFAST |
44.11 |
44.25 |
0.14 |
| 4 |
Wang, Sophia Y (9)
|
NIFAST |
46.79 |
45.33 |
-1.46 |
| 5 |
Blanchard, Zachary E (11)
|
NIFAST |
52.99 |
52.90 |
-0.09 |
| 6 |
Ferrari, Abigail C (10)
|
NIFAST |
|
53.03 |
- |
| 7 |
Comstra, Connor D (9)
|
NIFAST |
|
58.94 |
- |
| 8 |
Galley, Alyssa E (10)
|
NIFAST |
56.93 |
1:04.69 |
7.76 |
| 9 |
Schwartzbeck, Elisabeth M (11)
|
NIFAST |
|
1:13.95 |
- |
|
Shaw, Fiona M (10)
|
NIFAST |
|
DQ |
- |
|
Togay, Erdem A (10)
|
NIFAST |
1:00.97 |
DQ |
- |
|
Balland, Megan E (10)
|
NIFAST |
58.31 |
NS |
- |