| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Knight, Madeline P (17)
|
NISEYS |
25.27 |
25.99 |
0.72 |
| 2 |
Brady, Lauren V (15)
|
NIFAST |
27.31 |
26.58 |
-0.73 |
| 3 |
Kanaley, Grace M (14)
|
NISEYS |
26.58 |
26.78 |
0.20 |
| 4 |
Van Vreede, Olivia L (12)
|
NISEYS |
27.65 |
27.32 |
-0.33 |
| 5 |
Crye, Joleigh C (12)
|
NIFAST |
26.89 |
27.47 |
0.58 |
| 6 |
Ulatowski, Scarlett D (12)
|
NIFAST |
28.55 |
27.55 |
-1.00 |
| 7 |
Ritter, Anne (12)
|
NIPACK |
28.02 |
28.83 |
0.81 |
| 8 |
Danesh, Anna E (16)
|
NIFAST |
28.17 |
29.01 |
0.84 |
| 9 |
Sifling, Malia D (13)
|
NIPACK |
29.60 |
29.04 |
-0.56 |
| 10 |
Tucker, Abby C (13)
|
NIFAST |
29.02 |
29.63 |
0.61 |
| 11 |
Ganesan, Aruna (15)
|
NIFAST |
30.97 |
31.30 |
0.33 |
| 12 |
Sahin, Melis (12)
|
NIPACK |
31.67 |
32.43 |
0.76 |
| 13 |
Sotgiu, Isabella V (12)
|
NIFAST |
|
38.22 |
- |