| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Conley, Michael T (12)
|
NIFAST |
45.61 |
41.83 |
-3.78 |
| 2 |
Carter, Owen T (11)
|
NIFAST |
42.62 |
42.52 |
-0.10 |
| 3 |
Gallegos, Lena P (12)
|
NIFAST |
45.21 |
45.75 |
0.54 |
| 4 |
Wang, Sophia Y (9)
|
NIFAST |
45.74 |
46.33 |
0.59 |
| 5 |
Lawrence, Taylor B (12)
|
NIFAST |
46.80 |
49.34 |
2.54 |
| 6 |
Webb, Karen R (11)
|
NIFAST |
49.18 |
50.58 |
1.40 |
| 7 |
Kelley, Gage T (10)
|
NIFAST |
50.35 |
53.01 |
2.66 |
| 8 |
Sanders, Nina P (10)
|
NIFAST |
54.32 |
54.90 |
0.58 |
| 9 |
Ferrari, Abigail C (10)
|
NIFAST |
|
55.72 |
- |
| 10 |
Little, Mikayla L (11)
|
NIFAST |
1:12.59 |
57.90 |
-14.69 |
| 11 |
Togay, Erdem A (10)
|
NIFAST |
56.03 |
58.08 |
2.05 |
| 12 |
Galley, Alyssa E (10)
|
NIFAST |
1:11.21 |
1:04.39 |
-6.82 |
|
Donnaruma, Logan A (9)
|
NIFAST |
|
DQ |
- |
|
Laurence, Bridget K (10)
|
NIFAST |
1:06.27 |
DQ |
- |
|
Schwartzbeck, Elisabeth M (11)
|
NIFAST |
|
DQ |
- |