| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Obuhanych, Zachary J (11)
|
NIFAST |
40.24 |
40.69 |
0.45 |
| 2 |
Hartley, Elliana G (8)
|
NIFAST |
42.85 |
41.33 |
-1.52 |
| 3 |
Cardona, Lucia M (12)
|
NIFAST |
46.00 |
45.22 |
-0.78 |
| 4 |
Black, Meghan C (12)
|
NIFAST |
47.62 |
48.61 |
0.99 |
| 5 |
Casler, Hannah R (9)
|
NIFAST |
50.06 |
49.26 |
-0.80 |
| 5 |
Nedelko, Gleb Y (13)
|
NIFAST |
51.47 |
49.26 |
-2.21 |
| 7 |
Conley, Michael T (11)
|
NIFAST |
52.93 |
51.11 |
-1.82 |
| 8 |
Visconte, Adriana E (10)
|
NIFAST |
52.57 |
52.81 |
0.24 |
| 9 |
Brett, Erin E (9)
|
NIFAST |
52.76 |
53.01 |
0.25 |
| 10 |
Huerta-Andrade, Isabella (11)
|
NIFAST |
56.54 |
53.34 |
-3.20 |
| 11 |
Larzelere, Natalie E (9)
|
NIFAST |
57.00 |
57.25 |
0.25 |
| 12 |
Webb, Karen R (10)
|
NIFAST |
54.38 |
57.91 |
3.53 |
| 13 |
Montaque, Angelina M (10)
|
NIFAST |
53.25 |
58.06 |
4.81 |
| 14 |
Togay, Erdem A (9)
|
NIFAST |
1:07.47 |
1:01.27 |
-6.20 |
| 15 |
Cougle, Adam J (11)
|
NIFAST |
|
1:06.69 |
- |
| 16 |
Sanders, Nina P (10)
|
NIFAST |
1:04.51 |
1:09.63 |
5.12 |
| 17 |
Fortuna, Katelyn G (8)
|
NIFAST |
1:10.03 |
1:13.25 |
3.22 |
| 18 |
Scherlein, Parker J (13)
|
NIFAST |
|
1:16.36 |
- |
| 19 |
Galley, Alyssa E (9)
|
NIFAST |
|
1:17.18 |
- |
|
Hellman, Aidan S (13)
|
NIFAST |
43.00 |
DQ |
- |
|
Pound, Miranda M (13)
|
NIFAST |
|
DQ |
- |