| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Menon, Anoushka (12)
|
NTMTRO |
41.45 |
39.60 |
-1.85 |
| 2 |
Searle, Hailey G (11)
|
NTMTRO |
42.44 |
41.62 |
-0.82 |
| 3 |
Wallace, Brenna R (12)
|
NTMTRO |
47.28 |
43.42 |
-3.86 |
| 4 |
Gable, Sarah M (12)
|
NTMTRO |
45.44 |
43.57 |
-1.87 |
| 5 |
Tufail, Shanzay F (11)
|
NTUN |
41.88 |
44.39 |
2.51 |
| 6 |
Osmer, Britton S (11)
|
NTMTRO |
45.63 |
47.30 |
1.67 |
| 7 |
Sanderford, Bethany L (12)
|
NTMTRO |
47.70 |
47.88 |
0.18 |
| 8 |
Simmons, Reese N (11)
|
NTMTRO |
|
54.40 |
- |
| 9 |
Miller, Evie R (12)
|
NTMTRO |
|
57.64 |
- |
| 10 |
Sanderford, Elizabeth S (12)
|
NTMTRO |
59.58 |
58.44 |
-1.14 |
|
Wallace, Ashby E (12)
|
NTMTRO |
1:11.11 |
DQ |
- |