| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stoecker, Jules R (10)
|
NTMTRO |
31.46 |
32.72 |
1.26 |
| 2 |
Craver, Cayna R (9)
|
NTMTRO |
34.67 |
35.45 |
0.78 |
| 3 |
Upchurch, Lyla A (10)
|
NTMTRO |
38.90 |
36.80 |
-2.10 |
| 4 |
Agnew, Madison M (10)
|
NTMTRO |
40.27 |
37.80 |
-2.47 |
| 5 |
Gamage, Sasmi N (9)
|
NTMTRO |
42.29 |
39.77 |
-2.52 |
| 6 |
Xanthos, Jordan (8)
|
NTMTRO |
45.42 |
40.97 |
-4.45 |
| 7 |
Craver, Callie R (7)
|
NTMTRO |
48.38 |
46.46 |
-1.92 |
| 8 |
Daigle, Riley M (9)
|
NTMTRO |
|
47.30 |
- |
| 9 |
Price, Nora M (7)
|
NTMTRO |
|
54.23 |
- |
| 10 |
Spates, Ava O (10)
|
NTMTRO |
|
55.17 |
- |
|
Ismanto, Evangeline M (7)
|
NTMTRO |
|
NS |
- |
|
Sessions, Sofia L (10)
|
NTMTRO |
47.20 |
NS |
- |