| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lonergan, Avery P (12)
|
NTMTRO |
27.12 |
26.35 |
-0.77 |
| 2 |
Gamage, Venuki L (12)
|
NTMTRO |
30.00 |
29.62 |
-0.38 |
| 3 |
Snyder, Naomi G (11)
|
NTMTRO |
34.63 |
33.52 |
-1.11 |
| 4 |
Mejia, Madison T (11)
|
NTMTRO |
34.60 |
34.73 |
0.13 |
| 5 |
Sanderford, Elizabeth S (12)
|
NTMTRO |
38.69 |
34.76 |
-3.93 |
| 6 |
Hyland, Annabelle E (11)
|
NTMTRO |
37.66 |
35.03 |
-2.63 |
| 7 |
Sanderford, Bethany L (12)
|
NTMTRO |
37.46 |
35.31 |
-2.15 |
| 8 |
Osmer, Britton S (11)
|
NTMTRO |
35.38 |
35.34 |
-0.04 |
| 9 |
Ort, Katelyn J (12)
|
NTMTRO |
|
37.50 |
- |
| 10 |
Reinke, Embyr D (12)
|
NTMTRO |
|
50.22 |
- |
|
Jenkins, Samantha J (11)
|
NTMTRO |
42.14 |
NS |
- |