| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Huang, Yining (6)
|
PVFISH |
28.76 |
23.94 |
-4.82 |
| 2 |
Alsina, Amelia (6)
|
PVRMSC |
23.88 |
24.90 |
1.02 |
| 3 |
Dmitrova, Stephania Z (6)
|
PVFISH |
31.13 |
25.42 |
-5.71 |
| 4 |
Luongo, Blake A (6)
|
PVNCAP |
27.57 |
26.07 |
-1.50 |
| 5 |
Wang, Katherine A (6)
|
PVASA |
28.19 |
26.27 |
-1.92 |
| 6 |
Cai, Ashley A (6)
|
PVRMSC |
27.32 |
26.41 |
-0.91 |
| 7 |
Abrams, Ellis K (6)
|
PVRMSC |
29.18 |
26.52 |
-2.66 |
| 8 |
DeBacker, Sophie R (6)
|
PVRMSC |
26.09 |
26.79 |
0.70 |
| 9 |
Nielsen, Maddie G (6)
|
PVYORK |
|
27.45 |
- |
| 10 |
Parks, Erin E (6)
|
PVYORK |
31.26 |
27.71 |
-3.55 |
| 11 |
Mara, Madeline A (6)
|
PVPM |
25.88 |
28.10 |
2.22 |
| 12 |
Chavez, Patricia C (6)
|
PVRMSC |
30.82 |
28.16 |
-2.66 |
| 13 |
Cully, Camryn E (6)
|
PVYORK |
28.13 |
28.33 |
0.20 |
| 14 |
Ulen, Madeline G (6)
|
PVYORK |
27.59 |
28.82 |
1.23 |
| 15 |
Wofford, Maven A (6)
|
PVRMSC |
32.48 |
29.77 |
-2.71 |
| 16 |
Hansel, Lila M (6)
|
PVASA |
30.57 |
29.90 |
-0.67 |
| 17 |
Wheatley, Mays D (6)
|
PVFISH |
32.64 |
29.92 |
-2.72 |
| 18 |
Peterson, Lana B (6)
|
PVRMSC |
31.73 |
30.32 |
-1.41 |
| 19 |
Combet, Emma (6)
|
PVRMSC |
34.44 |
30.39 |
-4.05 |
| 20 |
Trepkowski, Sabrina N (6)
|
PVRMSC |
28.91 |
30.67 |
1.76 |
| 21 |
Lee, Olivia M (5)
|
PVRMSC |
32.97 |
30.75 |
-2.22 |
| 22 |
Eschinger, Lucy (6)
|
PVRMSC |
42.30 |
31.66 |
-10.64 |
| 23 |
Hyde, Anna L (6)
|
PVFBST |
|
32.97 |
- |
| 24 |
Verderese, Emeline S (6)
|
PVRMSC |
30.40 |
33.24 |
2.84 |
| 25 |
Wilson-Segovia, Suzannah A (6)
|
PVFBST |
|
35.96 |
- |
|
Drozd, Lesia O (6)
|
PVASA |
|
DQ |
- |
|
McNamara, Skyler J (6)
|
PVPM |
30.48 |
NS |
- |
|
Francis, Caroline V (6)
|
PVNCAP |
31.85 |
NS |
- |
|
Chubb, Felicity P (5)
|
PVYORK |
36.46 |
NS |
- |