| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Powell, Alyssa V (17)
|
CASBSC |
23.93 |
23.71 |
-0.22 |
| 2 |
Aycock, Courtney T (17)
|
SETNAQ |
23.95 |
23.75 |
-0.20 |
| 3 |
Melnick, Melissa E (15)
|
OZCSP |
23.57 |
23.94 |
0.37 |
| 4 |
Taylor, Danielle M (17)
|
PCWCAB |
23.80 |
24.01 |
0.21 |
| 5 |
Hatch, Jillian (16)
|
CCTNT |
24.14 |
24.03 |
-0.11 |
| 6 |
Steffens, Cailey M (17)
|
PCCROW |
23.53 |
24.05 |
0.52 |
| 7 |
Boles, Catherine I (16)
|
PCUN-0 |
24.20 |
24.11 |
-0.09 |
| 8 |
Hogg, Molly M (15)
|
SDASC |
23.97 |
24.16 |
0.19 |
| 9 |
Broome, Emma L (16)
|
AZSAC |
24.34 |
24.27 |
-0.07 |
| 10 |
Baynes-Tadena, Julienne P (17)
|
SNSMST |
23.48 |
24.33 |
0.85 |
| 11 |
Quintana, Heaven L (16)
|
SNWAC |
25.77 |
24.42 |
-1.35 |
| 12 |
Kosinski, Kathryn M (16)
|
GUPEAK |
24.17 |
24.44 |
0.27 |
| 13 |
Darham, Mira H (16)
|
MTMAC |
23.99 |
24.47 |
0.48 |
| 13 |
Stone, Sarah K (17)
|
NTDM |
24.37 |
24.47 |
0.10 |
| 15 |
Federlin, Jade A (16)
|
STNTRO |
24.10 |
24.51 |
0.41 |
| 16 |
Saghafi, Ariana D (16)
|
PCNBA |
23.99 |
24.67 |
0.68 |
| 17 |
Gillentine, Harper B (17)
|
NTRACE |
24.26 |
24.70 |
0.44 |
| 17 |
Strathman, Madison E (17)
|
MVTST |
24.63 |
24.70 |
0.07 |
| 19 |
Hedrick, Samantha T (16)
|
IAPELL |
24.66 |
24.80 |
0.14 |
| 19 |
England, Elena R (15)
|
CACLSS |
25.04 |
24.80 |
-0.24 |
| 21 |
Boddiford, Ashley N (16)
|
FLBD |
24.90 |
24.90 |
0.00 |
| 22 |
Wheeler, Kaylee N (14)
|
KYCARD |
|
24.91 |
- |
| 23 |
Bocock, Emily E (16)
|
GUESA |
24.80 |
24.92 |
0.12 |
| 24 |
Kelley, Bridget T (17)
|
FLSTAR |
52.53 |
25.02 |
-27.51 |
| 24 |
Ward, Madison W (16)
|
PCWCAB |
|
25.02 |
- |
| 26 |
Turner, Makenna L (15)
|
PCCROW |
25.50 |
25.13 |
-0.37 |
| 27 |
Ubele, Sarah E (18)
|
NEGS |
24.80 |
25.20 |
0.40 |
| 28 |
Dickson, Carly E (15)
|
NEGS |
24.48 |
25.33 |
0.85 |
| 29 |
Hultquist, Emma K (13)
|
NTRACE |
25.14 |
25.38 |
0.24 |