| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mortenson, Katrina E (17)
|
CAALPH |
23.30 |
23.66 |
0.36 |
| 2 |
Ottem, Ellery A (14)
|
MNMAKO |
23.88 |
23.76 |
-0.12 |
| 3 |
Leary, Kate (17)
|
PNSMAC |
24.21 |
23.98 |
-0.23 |
| 4 |
Davis, Jessica S (17)
|
PCCROW |
24.09 |
24.01 |
-0.08 |
| 5 |
Murphy, Quinn E (16)
|
AZPSC |
24.03 |
24.02 |
-0.01 |
| 6 |
Beay, Alison M (16)
|
WIRA |
24.17 |
24.17 |
0.00 |
| 7 |
Bernier, Allison R (17)
|
NMCAQ |
24.30 |
24.22 |
-0.08 |
| 7 |
Sifferman, Stephanie E (15)
|
PCSCSC |
24.35 |
24.22 |
-0.13 |
| 9 |
Howe, Elliott G (16)
|
UTSDAT |
24.08 |
24.35 |
0.27 |
| 10 |
Clark, Madeline R (16)
|
MWGOAL |
24.19 |
24.39 |
0.20 |
| 11 |
Hay, Norah G (17)
|
GUKATY |
24.31 |
24.40 |
0.09 |
| 12 |
Baker, Kate R (16)
|
COHRA |
24.36 |
24.43 |
0.07 |
| 13 |
Craig, Winter G (18)
|
MNRIPT |
23.98 |
24.77 |
0.79 |
| 14 |
Mills, Malia S (17)
|
CANOVA |
23.15 |
24.83 |
1.68 |
| 15 |
Eng, Lauren E (16)
|
CAGSC |
25.12 |
25.10 |
-0.02 |
| 16 |
Furqueron, Abigail K (16)
|
IESWAT |
25.08 |
25.48 |
0.40 |