| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Qali, Abbas M (23)
|
SEBAMA |
54.50 |
54.38 |
-0.12 |
| 2 |
Dear, Adam W (20)
|
NCUN-0 |
54.86 |
54.80 |
-0.06 |
| 3 |
Assaad, Gamal (20)
|
USWU |
53.58 |
54.89 |
1.31 |
| 4 |
Pomajevich, Samuel R (17)
|
PVNCAP |
54.86 |
55.67 |
0.81 |
| 5 |
Soto Ramirez, Luis F (23)
|
USITES |
|
55.70 |
- |
| 6 |
Moyar, Jacob L (21)
|
VAUN-0 |
58.10 |
56.81 |
-1.29 |
| 7 |
Dols, Keanan M (17)
|
FLSYS |
57.30 |
57.14 |
-0.16 |
| 8 |
Dugan, Michael T (21)
|
NCUN-0 |
55.20 |
57.26 |
2.06 |
| 9 |
Spinazzola, Luca M (22)
|
CATROJ |
55.50 |
57.46 |
1.96 |
| 10 |
Hanson, Joshua E (21)
|
CCUN-0 |
56.32 |
57.51 |
1.19 |
| 11 |
White, Justin R (20)
|
ILUN-0 |
58.20 |
57.70 |
-0.50 |
| 12 |
Goethe, Jackson P (20)
|
MIMICH |
58.21 |
59.52 |
1.31 |