| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Eldridge, Raena L (18)
|
NTRACE |
57.05 |
55.50 |
-1.55 |
| 2 |
Berkoff, Katharine F (13)
|
MTMAC |
56.80 |
56.94 |
0.14 |
| 3 |
Jin, Sarah P (17)
|
PCDACA |
57.21 |
57.48 |
0.27 |
| 4 |
Brockley, Piper A (17)
|
PCSRN |
1:00.15 |
57.93 |
-2.22 |
| 5 |
Stelnicki, Loretta P (17)
|
ILPAC |
57.95 |
57.98 |
0.03 |
| 6 |
Pentlarge, Jessica A (15)
|
SINCA |
57.67 |
58.11 |
0.44 |
| 7 |
Geyer, Meredith M (15)
|
SEGST |
58.80 |
58.71 |
-0.09 |
| 8 |
Nentwich, Reagan N (16)
|
STAAAA |
1:00.36 |
58.79 |
-1.57 |
| 9 |
Goeders, Anyamarie J (14)
|
INMAKO |
58.92 |
59.17 |
0.25 |
| 10 |
Chopp, Abigail M (17)
|
CACSSC |
57.99 |
59.21 |
1.22 |
| 11 |
O'Brien, Ashleen S (14)
|
PCOAPB |
58.22 |
59.42 |
1.20 |
| 12 |
McElrath, Emma M (16)
|
ADALBS |
58.60 |
59.78 |
1.18 |
| 13 |
Menzmer, Elizabeth G (14)
|
SNDART |
1:00.35 |
1:00.12 |
-0.23 |
| 14 |
Wierzbicki, Jessica A (14)
|
IEVS |
59.61 |
1:00.63 |
1.02 |
| 15 |
Svitavsky, Summer A (16)
|
LELESD |
1:00.67 |
1:01.02 |
0.35 |
| 16 |
Conners, Emily A (17)
|
MNBLA |
1:00.00 |
1:01.30 |
1.30 |