| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chau, Calvin W (10)
|
MRCIY |
33.81 |
33.22 |
-0.59 |
| 2 |
Chang, Steven R (10)
|
MRFLY |
36.64 |
36.58 |
-0.06 |
| 3 |
Zhao, Colin E (10)
|
MRFLY |
39.10 |
37.61 |
-1.49 |
| 4 |
O'Sullivan, Kealan J (9)
|
MRCIY |
41.39 |
39.15 |
-2.24 |
| 5 |
Beaman, Shawn F (9)
|
MRYFD |
40.58 |
39.53 |
-1.05 |
| 6 |
Davis-Whittaker, Jose (9)
|
MRFLY |
39.33 |
39.67 |
0.34 |
| 7 |
Sanchez, Ronald (10)
|
MRRED |
39.78 |
39.90 |
0.12 |
| 8 |
Diaz, Adam C (10)
|
MRRED |
48.48 |
41.38 |
-7.10 |
| 9 |
Grissinger, John H (10)
|
MRYFD |
42.56 |
42.03 |
-0.53 |
| 10 |
Lim, James (9)
|
MRFLY |
47.07 |
44.88 |
-2.19 |
| 11 |
Adnet, Achilles (10)
|
MRMCBY |
42.95 |
45.22 |
2.27 |
| 12 |
Park, Ian (9)
|
MRCIY |
47.99 |
45.67 |
-2.32 |
| 13 |
Leung, Lance (9)
|
MRFLY |
|
46.51 |
- |
| 14 |
Haynes, Mark C (10)
|
MRCIY |
47.47 |
46.59 |
-0.88 |
| 15 |
Lee, Jayden M (9)
|
MRFLY |
45.33 |
47.03 |
1.70 |
| 16 |
Cordova, Christian M (10)
|
MRCIY |
38.17 |
47.04 |
8.87 |
| 17 |
Corelli, Rocco F (10)
|
MRMCBY |
44.86 |
47.26 |
2.40 |
| 18 |
Vasquez, Andrew S (10)
|
MRFLY |
47.48 |
47.47 |
-0.01 |
| 19 |
Diao, Alexander B (10)
|
MRFLY |
46.79 |
47.70 |
0.91 |
| 20 |
Amen, Hunter R (9)
|
MRYFD |
|
48.95 |
- |
| 21 |
Ng, Evan (9)
|
MRCIY |
55.36 |
49.94 |
-5.42 |
| 22 |
Shulyar, Stanislau Z (9)
|
MRMCBY |
1:01.33 |
51.22 |
-10.11 |
| 23 |
Dwyer, Daniel P (10)
|
MRCIY |
58.19 |
51.91 |
-6.28 |
| 24 |
Sharkey, Caleb (9)
|
MRYFD |
53.35 |
53.83 |
0.48 |
| 25 |
Shah, Nikhil M (9)
|
MRYFD |
|
54.78 |
- |
| 26 |
Dorr, Henry J (10)
|
MRYFD |
56.56 |
55.24 |
-1.32 |
| 27 |
Robinson, Jesse W (10)
|
MRMCBY |
|
58.62 |
- |
| 28 |
Elms, Preston M (8)
|
MRYFD |
|
1:00.73 |
- |
|
Gandhi, Krish S (9)
|
MRYFD |
|
DQ |
- |
|
Rosen, Alexander M (10)
|
MRYFD |
|
DQ |
- |