| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lin, Esther Y (14)
|
FGPCS |
26.11 |
27.20 |
1.09 |
| 2 |
McLermore, Mercy P (13)
|
UNAT |
|
27.62 |
- |
| 3 |
Smith, Hanna E (14)
|
FGPCS |
27.07 |
27.87 |
0.80 |
| 4 |
Jones, Lara R (13)
|
FGPCS |
28.80 |
29.07 |
0.27 |
| 5 |
Armbruster, Kaitlyn P (13)
|
FGPCS |
29.70 |
30.57 |
0.87 |
| 6 |
Trussell, Lillian A (13)
|
FGPCS |
30.18 |
31.66 |
1.48 |
| 7 |
Zlateva, Victoria A (14)
|
FGPCS |
32.83 |
31.71 |
-1.12 |
| 8 |
Bennett, Lorelei K (13)
|
FGPCS |
32.97 |
32.13 |
-0.84 |
| 9 |
Leete, Siera A (13)
|
FGSFTL |
34.34 |
33.22 |
-1.12 |
| 10 |
Coan, Maya O (13)
|
FGSFTL |
34.34 |
33.68 |
-0.66 |
| 11 |
Salce, Haylie (14)
|
FGSFTL |
33.98 |
34.92 |
0.94 |
| 12 |
Lujan-Rodriguez, Catalina (14)
|
FGMS |
|
37.46 |
- |
| 13 |
Postupack, Sophia (13)
|
FGMS |
|
37.78 |
- |
| 14 |
Mantilla, Alessia (14)
|
FGMS |
44.16 |
38.75 |
-5.41 |
| 15 |
Marquicio, Maria S (14)
|
FGSFTL |
42.54 |
38.95 |
-3.59 |
|
Marques, Agatha C (14)
|
FGSFTL |
33.99 |
NS |
- |