| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lee, Ryan T (10)
|
MANSC |
44.36 |
44.34 |
-0.02 |
| 1 |
Choy, Chandler (9)
|
MAUN |
|
47.79 |
- |
| 2 |
Cohen, Radek A (10)
|
MAWCAC |
46.16 |
44.47 |
-1.69 |
| 2 |
Young, Oliver B (10)
|
MASERP |
54.71 |
48.79 |
-5.92 |
| 3 |
VanBuskirk, Will (9)
|
MAUN |
49.94 |
48.90 |
-1.04 |
| 4 |
Huang, Aaron J (10)
|
MASERP |
56.95 |
53.88 |
-3.07 |
| 5 |
Izhakov, Gabriel (9)
|
MANSC |
1:02.56 |
56.27 |
-6.29 |
| 6 |
Greineder, Max X (9)
|
MAFSS |
|
57.80 |
- |
| 7 |
McElwain, Grayson T (9)
|
MAJRAY |
1:00.67 |
58.53 |
-2.14 |
| 8 |
gor, Aidan D (10)
|
MAGAAC |
1:03.76 |
1:03.57 |
-0.19 |
|
Cratin, Ace J (9)
|
MAGAAC |
|
1:02.22 |
- |
|
Candio, Nicholas (9)
|
MAGLEC |
|
DQ |
- |
|
Kerelashvili, Alexander S (10)
|
MANSC |
52.57 |
NS |
- |
|
Reznitchenko, Andrew (10)
|
MAORCA |
51.20 |
NS |
- |