| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
DeCardona, Ella G (12)
|
MAGAAC |
41.31 |
41.75 |
0.44 |
| 2 |
Baran, Georgia C (11)
|
MAGAAC |
41.98 |
42.21 |
0.23 |
| 3 |
Loomis, Kaitlyn M (12)
|
MACHEL |
|
42.61 |
- |
| 4 |
Boles, Karly J (11)
|
MAGAAC |
42.14 |
42.63 |
0.49 |
| 5 |
Freeman, Sarah E (11)
|
MAGAAC |
43.46 |
44.13 |
0.67 |
| 6 |
Abraham, Audrey (11)
|
MACHEL |
44.28 |
45.05 |
0.77 |
| 7 |
Seidman, Jordan K (11)
|
MAGAAC |
46.09 |
45.25 |
-0.84 |
| 8 |
Soroka, Michelina R (11)
|
MADCAA |
46.47 |
46.03 |
-0.44 |
| 9 |
Arulselvam, Subhiksha S (11)
|
MAGAAC |
52.07 |
47.76 |
-4.31 |
| 10 |
Gomberg, Ariah (11)
|
MACHEL |
1:01.68 |
1:09.98 |
8.30 |
|
Miller, Dylan S (12)
|
MACHEL |
43.09 |
DQ |
- |
|
Hendrickson, Liliana J (11)
|
MAEAAC |
44.64 |
NS |
- |
|
Luviano, Caitlin A (12)
|
MAUN |
37.59 |
NS |
- |