| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bowen, Marika Z (9)
|
MANSC |
40.38 |
40.58 |
0.20 |
| 1 |
Kohm, Gabriella E (10)
|
MAGAAC |
45.11 |
41.71 |
-3.40 |
| 1 |
Quigley, Makayla A (10)
|
MANSC |
|
42.71 |
- |
| 2 |
Dymek, Julia J (10)
|
MALMAC |
41.77 |
42.53 |
0.76 |
| 2 |
DeMartinis, Catharina (9)
|
MASERP |
45.99 |
42.67 |
-3.32 |
| 2 |
Jarantow, Alexandra K (9)
|
MAPENN |
48.77 |
46.56 |
-2.21 |
| 3 |
Wood, Raegan O (10)
|
MANSC |
44.80 |
44.55 |
-0.25 |
| 3 |
Williams, Mazie L (10)
|
MAGAAC |
51.93 |
50.29 |
-1.64 |
| 4 |
Song, Sophia (10)
|
MAGAAC |
45.53 |
45.72 |
0.19 |
| 4 |
Chudamani, Avni (9)
|
MALIFE |
55.01 |
51.45 |
-3.56 |
| 5 |
Taddeo, Giavanna C (10)
|
MALIFE |
47.51 |
51.57 |
4.06 |
| 6 |
Ethiraj, Sanjita (9)
|
MAT85 |
1:04.84 |
54.64 |
-10.20 |
| 7 |
McCrudden, Charlotte K (9)
|
MAGAAC |
1:00.54 |
55.85 |
-4.69 |
| 8 |
Karpinski, Genevieve F (10)
|
MASERP |
53.20 |
56.03 |
2.83 |
| 9 |
Hassan, Sumiya B (10)
|
MASERP |
|
1:12.21 |
- |