| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jordan, Benjamin K (11)
|
NIGVST |
35.35 |
32.76 |
-2.59 |
| 2 |
Mariotti, Joseph A (11)
|
NIGVST |
35.20 |
33.86 |
-1.34 |
| 3 |
Miller, Riley W (12)
|
NIAAAC |
34.16 |
34.05 |
-0.11 |
| 4 |
Green, Austin W (11)
|
NIAAAC |
|
37.51 |
- |
| 5 |
Schuman, Colton A (11)
|
NIEMAC |
40.30 |
38.32 |
-1.98 |
| 6 |
Burns, Ryan D (12)
|
NIGVST |
40.50 |
38.56 |
-1.94 |
| 7 |
Wilber, Elliott D (12)
|
NIEMAC |
40.33 |
38.93 |
-1.40 |
| 8 |
Riling, Jesse L (11)
|
NIFKVL |
41.23 |
39.49 |
-1.74 |
| 9 |
Knowles, Brody C (12)
|
NIPVSC |
34.77 |
39.85 |
5.08 |
| 10 |
Kaus, Hayden G (11)
|
NIGWST |
42.10 |
40.08 |
-2.02 |
| 11 |
Pfaffenbach, Kadin L (12)
|
NIPVSC |
40.66 |
42.91 |
2.25 |
| 12 |
Scanlon, Thomas J (12)
|
NIEMAC |
45.32 |
44.42 |
-0.90 |
| 13 |
Lindquist, Benjamin M (11)
|
NIPVSC |
46.15 |
47.87 |
1.72 |
| 14 |
Rugg, Matthew T (12)
|
NIPVSC |
45.60 |
50.13 |
4.53 |