| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Barasch, Allison M (14)
|
OHFMST |
1:01.55 |
1:02.19 |
0.64 |
| 2 |
Gano, Haley N (14)
|
OHFMST |
1:04.93 |
1:04.30 |
-0.63 |
| 3 |
Freeworth, Kendall L (13)
|
OHSYL |
1:06.07 |
1:05.56 |
-0.51 |
| 4 |
Stevenson, Channing K (13)
|
OHSOS |
1:07.76 |
1:07.25 |
-0.51 |
| 5 |
Freeman, Sylvia R (13)
|
OHFMST |
1:08.99 |
1:07.92 |
-1.07 |
| 6 |
Desmond, Madeline G (13)
|
OHGTAC |
1:08.38 |
1:08.86 |
0.48 |
| 7 |
Turner, Skyler R (13)
|
OHRSC |
1:11.45 |
1:08.99 |
-2.46 |
| 8 |
Heinze, Delaney A (13)
|
OHGTAC |
1:04.24 |
1:09.68 |
5.44 |
| 9 |
Blakely, Amelie C (14)
|
OHFMST |
1:03.05 |
1:09.81 |
6.76 |
| 10 |
Atherine, Eliza (13)
|
OHFMST |
1:07.49 |
1:09.84 |
2.35 |
| 11 |
Dankert, Mia E (13)
|
OHSYL |
1:18.38 |
1:11.52 |
-6.86 |
| 12 |
Harms, Anna C (13)
|
OHGTAC |
1:18.37 |
1:12.97 |
-5.40 |
| 13 |
Slivinski, Olivia N (13)
|
LELSSC |
1:10.37 |
1:14.92 |
4.55 |
| 14 |
Evans, Megan E (14)
|
OHSOS |
1:17.21 |
1:15.65 |
-1.56 |
| 15 |
Diller, Aubrey L (13)
|
OHFMST |
1:14.97 |
1:17.26 |
2.29 |
| 16 |
Baccus, Kaelah S (13)
|
OHGTAC |
1:22.87 |
1:17.78 |
-5.09 |
| 17 |
Tuori, Grace A (13)
|
OHSYL |
|
1:20.25 |
- |
| 18 |
Stenger, Caroline J (13)
|
LELSSC |
1:42.52 |
1:32.11 |
-10.41 |
|
Leatherman, Abigail O (13)
|
OHBGSC |
1:09.05 |
NS |
- |