| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Blakely, Madeline A (16)
|
OHFMST |
57.85 |
1:00.99 |
3.14 |
| 2 |
Cheatwood, Mia L (15)
|
OHGTAC |
58.10 |
1:01.89 |
3.79 |
| 3 |
Jurski, Joslyn N (15)
|
OHUN-O |
1:00.19 |
1:02.88 |
2.69 |
| 4 |
Schuld, Morgan H (18)
|
LELSSC |
58.59 |
1:04.23 |
5.64 |
| 5 |
Desmond, Michaela A (15)
|
OHGTAC |
1:00.66 |
1:05.06 |
4.40 |
| 6 |
Karabatsos, Margaret L (16)
|
LELSSC |
1:02.47 |
1:05.45 |
2.98 |
| 7 |
Chaffee, Paige F (17)
|
OHSYL |
1:02.24 |
1:05.86 |
3.62 |
| 8 |
Foster, Alaina K (18)
|
OHBGSC |
1:00.60 |
1:06.60 |
6.00 |
| 9 |
Spradlin, Delaney H (15)
|
OHFAST |
1:01.38 |
1:08.18 |
6.80 |
| 10 |
Crooks, Maya A (17)
|
OHSYL |
1:05.97 |
1:08.20 |
2.23 |
| 11 |
Druyor, Erin N (17)
|
OHRSC |
1:02.44 |
1:08.46 |
6.02 |
| 12 |
Brown, Mara E (16)
|
OHSOS |
1:04.91 |
1:09.13 |
4.22 |
| 13 |
Fisher, Kathryn R (16)
|
OHBGSC |
1:09.20 |
1:09.35 |
0.15 |
| 14 |
Russell, Ella K (15)
|
LELSSC |
1:04.42 |
1:09.70 |
5.28 |
| 15 |
Nawrocki, Grace C (18)
|
OHSYL |
1:03.58 |
1:09.73 |
6.15 |
| 16 |
Gross, Lily C (17)
|
LEVSC |
1:12.20 |
1:10.22 |
-1.98 |
| 17 |
Katsaras, Lillian M (15)
|
LEUN-L |
1:08.93 |
1:10.44 |
1.51 |
| 18 |
Haitonic, Rachel E (16)
|
OHSOS |
1:04.88 |
1:11.31 |
6.43 |
| 19 |
Briggs, Samantha J (15)
|
OHSYL |
1:08.12 |
1:11.56 |
3.44 |
| 20 |
Katayama, Klavdia M (17)
|
OHFAST |
1:00.41 |
1:14.67 |
14.26 |
| 21 |
Glowacki, Samantha L (17)
|
OHBGSC |
1:12.24 |
1:16.81 |
4.57 |
| 22 |
Storrer, Gabrielle M (15)
|
OHGTAC |
1:31.62 |
1:29.40 |
-2.22 |
| 23 |
Gase, Abigail C (17)
|
OHBGSC |
1:24.99 |
1:31.32 |
6.33 |
|
Kelly, Madison M (17)
|
LELSSC |
56.08 |
NS |
- |
|
Gilley, Lexi L (16)
|
OHGTAC |
1:01.42 |
NS |
- |
|
Smith, Leah D (15)
|
OHSYL |
1:09.09 |
NS |
- |