| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Karasinski, Gabriella (7)
|
MAPENN |
44.56 |
38.78 |
-5.78 |
| 2 |
Davidson, Aubrey E (7)
|
MAUN |
41.20 |
39.26 |
-1.94 |
| 3 |
Elko, Madelyn E (7)
|
MASEA |
40.82 |
40.08 |
-0.74 |
| 4 |
Devlin, Mia L (7)
|
MAWAC |
41.36 |
44.00 |
2.64 |
| 5 |
McKendrick, Ainsley K (7)
|
MATWAC |
|
44.11 |
- |
| 6 |
Agne, Olivia L (7)
|
MAWAC |
54.14 |
44.28 |
-9.86 |
| 7 |
Peretti, Victoria I (7)
|
MAGPAC |
|
45.04 |
- |
| 8 |
Cornejo, Rachel P (7)
|
MAPCAC |
59.44 |
46.10 |
-13.34 |
| 9 |
Kumar, Ianna K (7)
|
MAUMAC |
1:07.22 |
48.80 |
-18.42 |
| 10 |
Briscoe, Chanelle N (7)
|
MAGPAC |
|
49.24 |
- |
| 11 |
Hutton, Ava L (7)
|
MAWAC |
|
49.29 |
- |
| 12 |
Schroeder, Caroline K (7)
|
MAUN |
1:02.75 |
50.77 |
-11.98 |
| 13 |
Rice, Addison (7)
|
MAWAC |
|
52.03 |
- |
| 14 |
Nunez-Ledesma, Amelie (7)
|
MANSC |
|
52.49 |
- |
| 15 |
Colon, Karrington R (7)
|
MAGPAC |
|
55.12 |
- |
| 16 |
Sullivan, Delaney K (7)
|
MAWAC |
|
58.19 |
- |
| 17 |
Golden, Allison P (7)
|
MAWAC |
1:17.88 |
1:00.07 |
-17.81 |
| 18 |
Hays, Grace L (7)
|
MAECCC |
1:11.91 |
1:02.89 |
-9.02 |
| 19 |
Larish, Eva M (7)
|
MAPRSC |
|
1:03.73 |
- |
|
Smolenski, Lilly G (7)
|
MAWAP |
|
NS |
- |