| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Forst, Preston C (16)
|
NCEAC |
46.63 |
47.58 |
0.95 |
| 2 |
Burhans, Tucker F (18)
|
NCEAC |
45.28 |
47.64 |
2.36 |
| 3 |
Miller, Cameron W (17)
|
NCEAC |
47.93 |
49.64 |
1.71 |
| 4 |
Legge, Joshua W (17)
|
NCMOR |
48.48 |
50.05 |
1.57 |
| 5 |
Nannucci, Pietro (17)
|
NCSGSA |
47.28 |
50.15 |
2.87 |
| 6 |
Adams II, Jeffery A (17)
|
NCEAC |
47.51 |
50.31 |
2.80 |
| 7 |
Keaney, Reilly E (14)
|
NCSGSA |
50.76 |
51.73 |
0.97 |
| 8 |
Barden, Luke H (17)
|
NCSGSA |
49.31 |
52.07 |
2.76 |
| 9 |
Auderer, Michael A (16)
|
NCMOR |
49.45 |
53.07 |
3.62 |
| 10 |
Womble, Ethan V (15)
|
NCGCY |
53.68 |
53.20 |
-0.48 |
| 11 |
Muire, Eric N (16)
|
NCGCY |
51.54 |
53.70 |
2.16 |
| 12 |
Fernandez, Aaron F (17)
|
NCGCY |
51.07 |
53.71 |
2.64 |
| 13 |
Harris, Dax C (13)
|
NCGCY |
52.48 |
53.96 |
1.48 |
| 14 |
Van der Linden, Brandon E (17)
|
NCGCY |
51.39 |
54.14 |
2.75 |
| 15 |
Watkins, Griffin A (15)
|
NCEAC |
53.23 |
55.20 |
1.97 |
| 16 |
McDonald, Benjamin M (14)
|
NCGCY |
54.53 |
55.50 |
0.97 |
| 17 |
Mills, Brandon R (15)
|
NCEAC |
54.68 |
55.65 |
0.97 |
| 18 |
Gatling III, Ladaniel (13)
|
NCGCY |
54.98 |
55.72 |
0.74 |
| 19 |
Smelzer, Peter B (14)
|
NCSGSA |
56.64 |
55.73 |
-0.91 |
| 20 |
Peterson, Benjamin E (14)
|
NCSGSA |
1:00.00 |
57.44 |
-2.56 |
| 21 |
Walls, Harrison R (14)
|
NCEAC |
58.35 |
57.87 |
-0.48 |
| 22 |
Tate, Fletcher K (13)
|
NCGCY |
58.71 |
58.19 |
-0.52 |
| 23 |
Rock, Noah L (12)
|
NCGCY |
56.87 |
58.46 |
1.59 |
| 24 |
Brooks, Jason D (13)
|
NCGCY |
59.93 |
58.70 |
-1.23 |
| 25 |
Presson, William A (15)
|
NCGCY |
58.09 |
58.77 |
0.68 |
| 26 |
Morrow, Connor E (13)
|
NCEAC |
58.07 |
1:05.45 |
7.38 |
|
Mikuta, Reid C (16)
|
NCSGSA |
50.84 |
DQ |
- |
|
Trent, Thomas H (16)
|
NCSGSA |
49.48 |
NS |
- |