| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Edwards, Maxwell E (18)
|
NCEAC |
49.05 |
50.83 |
1.78 |
| 2 |
Hamlet, Thomas E (17)
|
NCEAC |
48.76 |
51.74 |
2.98 |
| 3 |
Langston, Mitchell B (17)
|
NCMOR |
51.02 |
53.81 |
2.79 |
| 4 |
Ssengonzi, Jesse S (16)
|
NCMOR |
58.99 |
53.82 |
-5.17 |
| 5 |
Tars, Robert M (15)
|
NCGCY |
52.60 |
54.79 |
2.19 |
| 6 |
Kring, Jaden S (17)
|
NCMOR |
52.47 |
54.84 |
2.37 |
| 7 |
Silver, Ryan J (15)
|
NCMOR |
52.09 |
55.05 |
2.96 |
| 8 |
Spieler, Robert E (17)
|
NCEAC |
55.43 |
57.69 |
2.26 |
| 9 |
O'Neill, Trevor M (16)
|
NCMOR |
54.88 |
58.21 |
3.33 |
| 10 |
Bridgham, Joey J (15)
|
NCMOR |
54.30 |
58.24 |
3.94 |
| 11 |
Coffield, Aidan C (14)
|
NCEAC |
1:02.75 |
1:00.28 |
-2.47 |
| 12 |
Hall, Charles Samuel (16)
|
NCMOR |
1:03.08 |
1:00.50 |
-2.58 |
| 13 |
Hering, Flinn M (15)
|
NCSGSA |
58.86 |
1:00.67 |
1.81 |
| 14 |
Copeland, Harrison B (16)
|
NCEAC |
1:02.64 |
1:02.44 |
-0.20 |
| 15 |
Brown, Logan R (15)
|
NCEAC |
1:00.83 |
1:03.25 |
2.42 |
| 16 |
Muire, Eric N (17)
|
NCGCY |
59.31 |
1:04.04 |
4.73 |
| 17 |
Parsons, Garreth A (17)
|
NCMOR |
1:00.57 |
1:04.80 |
4.23 |
| 18 |
Maxey, Stone H (13)
|
NCEAC |
1:02.79 |
1:05.12 |
2.33 |
| 19 |
Holian, Tanner J (13)
|
NCSGSA |
1:03.29 |
1:05.53 |
2.24 |
| 20 |
Seeber, Andrew C (13)
|
NCGCY |
1:09.61 |
1:07.86 |
-1.75 |
|
Kwon, Kyle I (14)
|
NCGCY |
59.43 |
NS |
- |
|
Guthrie, Wyatt G (17)
|
NCGCY |
57.01 |
NS |
- |