| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Liu, William Z (12)
|
OHWOR |
3:07.76 |
2:47.47 |
-20.29 |
| 2 |
Ament, Logan J (12)
|
OHWOR |
3:18.24 |
2:52.19 |
-26.05 |
| 3 |
Wion, Owen J (11)
|
OHWAC |
3:02.85 |
2:57.11 |
-5.74 |
| 4 |
West, Lincoln D (12)
|
OHBKYS |
3:08.88 |
2:57.59 |
-11.29 |
| 5 |
DaJusta, Samir Daniel S (12)
|
OHUASC |
3:45.00 |
3:00.39 |
-44.61 |
| 6 |
Masters, Frank L (11)
|
OHUASC |
3:12.18 |
3:04.44 |
-7.74 |
| 7 |
Bosler, Charles P (12)
|
OHWAC |
2:56.30 |
3:09.37 |
13.07 |
| 8 |
Sommer, Jack S (12)
|
OHUASC |
4:00.00 |
3:10.44 |
-49.56 |
| 9 |
Cline, Patrick J (12)
|
OHWOR |
3:10.00 |
3:15.34 |
5.34 |
| 10 |
Neininger, Gavin J (11)
|
OHBKYS |
|
3:16.21 |
- |
| 11 |
Rumburg, James L (12)
|
OHLTPY |
3:16.99 |
3:18.66 |
1.67 |
| 12 |
Wolfe, Levi S (11)
|
OHWAC |
3:32.76 |
3:19.83 |
-12.93 |
| 13 |
Francisco, Luca V (11)
|
OHWAC |
3:48.51 |
3:24.40 |
-24.11 |
| 14 |
Yang, Cliff (11)
|
OHBKYS |
3:27.79 |
3:25.64 |
-2.15 |
| 15 |
Caldwell, Cameron E (11)
|
OHLTPY |
3:34.00 |
3:26.18 |
-7.82 |
| 16 |
Moisey, Jaden T (12)
|
OHGCST |
|
3:49.32 |
- |
| 17 |
Spector, Nathaniel J (12)
|
OHGCST |
|
4:11.91 |
- |
| 18 |
Lickfelt, Jacob K (11)
|
OHLTPY |
4:02.00 |
4:34.86 |
32.86 |
| 19 |
Zemmouri, Ouail (11)
|
OHLTPY |
4:45.67 |
4:49.81 |
4.14 |
|
Ebeidy, Yassin B (12)
|
OHBKYS |
3:26.32 |
NS |
- |
|
Trombold, William E (11)
|
OHUASC |
4:00.00 |
NS |
- |
|
Wanke, Noah N (12)
|
OHUASC |
4:00.00 |
NS |
- |