| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kovatch, Kieran G (10)
|
OHBKYS |
38.59 |
42.33 |
3.74 |
| 2 |
Sagle, Tyler J (9)
|
OHWOR |
53.61 |
44.44 |
-9.17 |
| 3 |
Alisiswanto, Joshua I (10)
|
OHBKYS |
1:04.75 |
47.07 |
-17.68 |
| 4 |
Kourie, Lucas D (10)
|
OHWOR |
58.00 |
50.89 |
-7.11 |
| 5 |
Bollinger, Beckett A (9)
|
OHBKYS |
49.40 |
55.38 |
5.98 |
| 6 |
Rumburg, Brennan A (10)
|
OHLTPY |
48.52 |
55.72 |
7.20 |
| 7 |
Moretti-Slaven, Trey D (9)
|
OHWAC |
57.79 |
1:00.21 |
2.42 |
| 8 |
Ebeidy, Adam B (9)
|
OHBKYS |
1:00.38 |
1:00.32 |
-0.06 |
| 9 |
Zhang, Aiden H (9)
|
OHLTPY |
51.00 |
1:00.63 |
9.63 |
| 10 |
Gonzalez Diaz, Hugo R (10)
|
OHWOR |
1:10.00 |
1:03.33 |
-6.67 |
| 11 |
Bogner, Dayne P (9)
|
OHWOR |
1:00.00 |
1:06.69 |
6.69 |
| 12 |
Campana, Dominic J (10)
|
OHWOR |
1:10.93 |
1:08.83 |
-2.10 |
|
Giusto, Giuseppe (10)
|
OHBKYS |
1:10.00 |
DQ |
- |
|
McNair, Robert O (9)
|
OHGCST |
|
DQ |
- |
|
Rucker, John R (9)
|
OHUASC |
1:22.00 |
DQ |
- |