| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bloss, Maura E (12)
|
MYST |
30.39 |
30.50 |
0.11 |
| 2 |
Riehl, Claire E (11)
|
MYST |
35.00 |
32.83 |
-2.17 |
| 3 |
Whitlow, Mallory J (12)
|
NIBRAD |
33.22 |
33.58 |
0.36 |
| 4 |
Swain, Cassidy L (11)
|
COUN |
34.41 |
33.78 |
-0.63 |
| 5 |
Smith, Kallie (12)
|
OCY |
35.17 |
34.35 |
-0.82 |
| 6 |
Gracey-Dalton, Kendall L (11)
|
NIBRAD |
35.57 |
35.49 |
-0.08 |
| 7 |
Kelley, Brooke (12)
|
TYST |
36.48 |
36.42 |
-0.06 |
| 8 |
Colwell, Sara (11)
|
COR |
35.82 |
36.52 |
0.70 |
| 9 |
Bennett, Samantha M (12)
|
COUN |
37.81 |
36.62 |
-1.19 |
| 10 |
Bland, Skyler L (11)
|
MYST |
38.22 |
36.64 |
-1.58 |
| 11 |
Quinn, Katherine E (11)
|
COUN |
35.30 |
36.68 |
1.38 |
| 12 |
Faulk, Morgan (11)
|
YJET |
37.28 |
36.86 |
-0.42 |
| 13 |
Foradori, Madeline V (12)
|
COUN |
36.32 |
36.89 |
0.57 |
| 14 |
Hart, Kelly (11)
|
ZZESYS |
39.18 |
38.76 |
-0.42 |
| 15 |
Brook, Eliza (11)
|
WRN |
18.27 |
38.97 |
20.70 |
| 16 |
Walker, Madeline (11)
|
WRN |
40.16 |
39.23 |
-0.93 |
| 17 |
Burch, Chelsea (11)
|
ZZESYS |
40.63 |
39.66 |
-0.97 |
| 18 |
McAvoy, Elsa (12)
|
YJET |
38.69 |
39.82 |
1.13 |
| 19 |
Mucha, Olivia (11)
|
TYST |
40.09 |
40.32 |
0.23 |
| 20 |
Fennick, Brea (12)
|
OCY |
41.48 |
41.09 |
-0.39 |
| 21 |
Campogiani, Adriana (11)
|
NIBRAD |
42.41 |
43.41 |
1.00 |
| 22 |
Crawford, Paisley (12)
|
TYST |
44.33 |
44.43 |
0.10 |
| 23 |
Wessell, Morgan (11)
|
OCY |
45.55 |
45.20 |
-0.35 |
| 24 |
Kirvan, Flynn (11)
|
TYST |
45.29 |
45.29 |
0.00 |
| 25 |
Lumia, Elsa (11)
|
YJET |
45.57 |
46.02 |
0.45 |
| 26 |
Hess, Ali (11)
|
COUN |
52.08 |
51.00 |
-1.08 |
| 27 |
Lander, Eden (11)
|
ZZESYS |
52.15 |
52.14 |
-0.01 |
| 28 |
Marshall, Makiraha (12)
|
YJET |
1:20.20 |
1:09.13 |
-11.07 |
|
Gindlesperger, Daphne C (11)
|
COUN |
42.34 |
DQ |
- |