| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Eroh, Natalie S (14)
|
AMRGSY |
25.39 |
25.22 |
-0.17 |
| 2 |
Burns, Ann-Katherine (14)
|
AMBYAC |
27.48 |
25.88 |
-1.60 |
| 3 |
Schollaert, Therese A (14)
|
AMSPY |
26.44 |
25.95 |
-0.49 |
| 4 |
Carson, Lena (14)
|
ZZTLYZ |
25.99 |
26.86 |
0.87 |
| 5 |
Fraundorfer, Alexis R (14)
|
AMBRY |
28.13 |
27.21 |
-0.92 |
| 6 |
Altman, Mary Anne (13)
|
MVY |
27.10 |
27.40 |
0.30 |
| 7 |
Solich, Nora A (14)
|
AMRESY |
27.92 |
27.57 |
-0.35 |
| 8 |
Stodolsky, Abigale (13)
|
LWCY |
27.89 |
27.65 |
-0.24 |
| 9 |
Kubitza, Carley (14)
|
MVY |
27.96 |
27.69 |
-0.27 |
| 10 |
Woltjen, Alexandra R (13)
|
AMSPY |
28.49 |
27.98 |
-0.51 |
| 11 |
Williams, Kasey L (13)
|
AMGYST |
28.45 |
28.35 |
-0.10 |
| 12 |
Pflugh, Emma (14)
|
AMBYAC |
28.24 |
28.38 |
0.14 |
| 13 |
Flatt, Brynn V (13)
|
AMRESY |
28.33 |
28.44 |
0.11 |
| 14 |
Novotnak, Kaylie (13)
|
MVY |
28.24 |
29.12 |
0.88 |
| 15 |
Stipanovich, Loren B (13)
|
AMBRY |
28.12 |
29.86 |
1.74 |