| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shackley, Leah E (14)
|
AMBRY |
57.23 |
59.88 |
2.65 |
| 2 |
Ricciuti, Giuliana L (13)
|
AMSPY |
1:02.09 |
1:00.54 |
-1.55 |
| 3 |
Connelly, Reagan E (13)
|
AMRESY |
1:03.92 |
1:03.32 |
-0.60 |
| 4 |
Smitley, Mikayla (14)
|
LVY |
1:03.76 |
1:03.38 |
-0.38 |
| 5 |
Vance, Abigail E (13)
|
AMBYAC |
1:08.93 |
1:06.99 |
-1.94 |
| 6 |
Kohan, Karlee E (14)
|
AMGJY |
1:10.00 |
1:08.06 |
-1.94 |
| 7 |
Schrock, Olivia C (13)
|
AMGYST |
1:09.94 |
1:08.17 |
-1.77 |
| 8 |
Traggiai, Addyson (13)
|
AMBYAC |
1:11.13 |
1:09.16 |
-1.97 |
| 9 |
Novotnak, Kaylie (13)
|
MVY |
1:10.46 |
1:11.36 |
0.90 |
|
Stipanovich, Loren B (13)
|
AMBRY |
1:10.47 |
DQ |
- |