| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Brown, Reegan A (15)
|
AMGYST |
59.31 |
1:00.02 |
0.71 |
| 1 |
Yurkovich, Hailey L (16)
|
AMUNTY |
59.71 |
1:00.02 |
0.31 |
| 3 |
Connelly, Riley A (16)
|
AMRESY |
1:00.42 |
1:00.10 |
-0.32 |
| 4 |
Barton, Ava B (15)
|
AMRGSY |
1:02.69 |
1:01.95 |
-0.74 |
| 5 |
Carmazzi, Brittney A (18)
|
AMRESY |
1:02.06 |
1:02.39 |
0.33 |
| 6 |
Pribozie, Ariel E (16)
|
AMGJY |
1:02.58 |
1:05.29 |
2.71 |
| 7 |
Andrews, Sidney (16)
|
LWCY |
1:05.73 |
1:05.82 |
0.09 |
| 8 |
Guinther, Brianna (17)
|
AMGYST |
1:07.74 |
1:06.52 |
-1.22 |
| 9 |
Pellegrino, Isabella M (16)
|
AMRESY |
1:07.59 |
1:06.64 |
-0.95 |
| 10 |
Fraundorfer, Emma L (15)
|
AMBRY |
1:07.60 |
1:07.58 |
-0.02 |
| 11 |
Olshanski, Alyssa (17)
|
AMBYAC |
1:06.95 |
1:07.98 |
1.03 |
| 12 |
Fanella, Autumn H (17)
|
INDY |
1:05.20 |
1:08.07 |
2.87 |
| 13 |
Lamb, Lily (17)
|
LWCY |
1:07.28 |
1:09.00 |
1.72 |
| 14 |
Sullivan, Emma J (15)
|
AMSPY |
1:08.19 |
1:09.30 |
1.11 |
| 15 |
Wilson, Skyler P (17)
|
AMUNTY |
1:07.43 |
1:09.70 |
2.27 |
| 16 |
DiMaio, Eva G (15)
|
AMRGSY |
1:07.38 |
1:11.19 |
3.81 |