| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pan, Amy S (12)
|
MIFFYS |
1:00.01 |
59.90 |
-0.11 |
| 2 |
Fisher, Katherine A (12)
|
OHCY |
1:01.95 |
1:02.03 |
0.08 |
| 3 |
Zyniewicz, Adele (12)
|
INYMMI |
1:02.35 |
1:02.08 |
-0.27 |
| 4 |
Khan, Asma N (12)
|
LERYD |
1:04.59 |
1:03.16 |
-1.43 |
| 5 |
Elgin, Olivia R (12)
|
LEGYB |
1:04.61 |
1:03.63 |
-0.98 |
| 6 |
Johnson, Alexandra F (12)
|
OHABLY |
1:04.00 |
1:04.14 |
0.14 |
| 7 |
Vincent, Cadence E (12)
|
WVRAYS |
1:04.80 |
1:04.59 |
-0.21 |
| 8 |
Markley, Grace I (12)
|
OHLAKY |
1:04.68 |
1:04.89 |
0.21 |
| 9 |
Matern, Sofia E (11)
|
OHABLY |
1:06.58 |
1:04.90 |
-1.68 |
| 10 |
Dezelic, Mia (12)
|
LECFYN |
1:05.58 |
1:05.43 |
-0.15 |
| 11 |
Blakely, Amelie C (12)
|
OHFMST |
1:06.19 |
1:05.47 |
-0.72 |
| 12 |
Lewis, Anna V (12)
|
OHBASH |
1:06.82 |
1:06.08 |
-0.74 |
| 13 |
Hurley, Anika S (11)
|
OHMCY |
1:08.32 |
1:06.57 |
-1.75 |
| 14 |
Norman, Anna L (12)
|
AMWHY |
1:07.81 |
1:06.61 |
-1.20 |
| 15 |
Vaulx, Alison C (11)
|
OHDRY |
1:06.94 |
1:06.78 |
-0.16 |
| 16 |
Niekamp, Kylie (12)
|
ZZAMFY |
1:07.14 |
1:06.93 |
-0.21 |
| 17 |
Chu, Kylene R (12)
|
OHPCY |
1:07.86 |
1:07.28 |
-0.58 |
| 18 |
Frazee, Sofi (12)
|
INYMMI |
1:06.86 |
1:07.36 |
0.50 |
| 19 |
Swallow, Katherine R (12)
|
MIFFYS |
1:07.59 |
1:07.91 |
0.32 |
| 20 |
Mitchell, Lily M (11)
|
OHABLY |
1:07.96 |
1:08.05 |
0.09 |
| 21 |
Parrish, Maristella G (12)
|
LEGO |
1:07.93 |
1:08.54 |
0.61 |
| 22 |
Benedetti, Aleia M (12)
|
MIMYM |
1:06.24 |
1:08.63 |
2.39 |
| 23 |
Montgomery, Emma (11)
|
SHLB |
1:07.49 |
1:08.82 |
1.33 |
| 24 |
Nixon, Annabelle (12)
|
NCY |
1:06.63 |
1:09.39 |
2.76 |
| 25 |
Wilcher, Ashlee L (12)
|
WVWILD |
1:07.89 |
1:10.15 |
2.26 |
| 26 |
Smith, Annalise M (12)
|
OHBASH |
1:07.65 |
1:10.36 |
2.71 |