| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zuliani, Carter (10)
|
WVRAYS |
1:03.31 |
1:01.15 |
-2.16 |
| 2 |
Short, John O (10)
|
OHCY |
1:02.70 |
1:01.42 |
-1.28 |
| 3 |
Cooper, Owen T (10)
|
MIFLY |
1:02.59 |
1:03.05 |
0.46 |
| 4 |
Guthrie, Owen (10)
|
WVRAYS |
1:04.22 |
1:03.50 |
-0.72 |
| 5 |
Meyers, Hayden W (10)
|
OHABLY |
1:03.53 |
1:04.21 |
0.68 |
| 6 |
Bhavaraju, Shreeyans * (10)
|
LERYD |
1:05.17 |
1:04.39 |
-0.78 |
| 7 |
Triplett, Bradey (10)
|
ZZAMFY |
1:06.74 |
1:05.44 |
-1.30 |
| 8 |
MacKillop, Patrick S (10)
|
MIBBD |
1:07.30 |
1:05.93 |
-1.37 |
| 9 |
Newcomb, Jackson D (10)
|
ZZAMFY |
1:07.28 |
1:06.07 |
-1.21 |
| 10 |
Tseng, Chih-Hsiun A (10)
|
OHLTPY |
1:05.20 |
1:06.21 |
1.01 |
| 11 |
Walley, Brock R (10)
|
ZZAMFY |
1:04.75 |
1:06.47 |
1.72 |
| 12 |
Jordan, Ryan M (10)
|
OHYWSC |
1:05.23 |
1:06.50 |
1.27 |
| 13 |
Mignery, Luke E (9)
|
OHPCY |
1:06.74 |
1:06.79 |
0.05 |
| 14 |
Jiang, Andy (10)
|
ZZAMFY |
1:07.23 |
1:06.84 |
-0.39 |
| 15 |
Bush, Ethan A (10)
|
OHEAST |
1:07.41 |
1:07.06 |
-0.35 |
| 16 |
Kauffman, Steven V (10)
|
OHPCY |
1:05.30 |
1:07.34 |
2.04 |
| 17 |
Malouf, Jonathan R (9)
|
OHLTPY |
1:07.47 |
1:07.54 |
0.07 |
| 18 |
Julian, Wyatt A (10)
|
OHEAST |
1:07.30 |
1:10.31 |
3.01 |