| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mrosko, Maria J (16)
|
AMUNTY |
57.42 |
57.74 |
0.32 |
| 2 |
Webster, Laryssa G (15)
|
NIBRAD |
59.19 |
58.05 |
-1.14 |
| 3 |
Glasspool, Elizabeth M (16)
|
AMSPY |
1:00.44 |
59.89 |
-0.55 |
| 4 |
BecK, Zoey R (16)
|
AMGCY |
1:02.99 |
1:01.38 |
-1.61 |
| 5 |
Leskovansky, Abby (16)
|
CLFD |
1:01.86 |
1:01.48 |
-0.38 |
| 6 |
Moini, Julia S (16)
|
NIBRAD |
1:02.54 |
1:02.19 |
-0.35 |
| 7 |
Weiss, Ellie (18)
|
AMBYAC |
1:02.59 |
1:02.74 |
0.15 |
| 8 |
Patarini, Angelina (17)
|
AMRESY |
1:02.57 |
1:03.03 |
0.46 |
| 9 |
McAllister, Kate (17)
|
TYST |
1:04.36 |
1:03.64 |
-0.72 |
| 10 |
Glasspool, Abigail R (18)
|
AMSPY |
1:07.48 |
1:04.08 |
-3.40 |
| 11 |
Margita, Anna (15)
|
INDY |
1:05.14 |
1:04.16 |
-0.98 |
| 12 |
Elsey, Gwendolyn (16)
|
SVY |
1:06.55 |
1:04.43 |
-2.12 |
| 13 |
Dzurko, Madison V (17)
|
AMBRY |
1:03.69 |
1:04.48 |
0.79 |
| 14 |
Wenner, Dana (15)
|
OCY |
1:04.22 |
1:04.50 |
0.28 |
| 15 |
Li, Emmy (17)
|
NIBRAD |
1:06.86 |
1:05.27 |
-1.59 |
| 16 |
Peyton, Macie (18)
|
COUN |
1:06.59 |
1:05.97 |
-0.62 |
| 17 |
Wilson, Skyler P (16)
|
AMUNTY |
1:05.76 |
1:06.43 |
0.67 |
| 18 |
Forcellini, Cydney R (16)
|
AMGJY |
1:05.27 |
1:06.67 |
1.40 |
| 19 |
Maloy, Molly A (17)
|
AMUNTY |
1:06.55 |
1:07.44 |
0.89 |
| 20 |
Wood, Jaylin (15)
|
CLFD |
1:07.97 |
1:09.17 |
1.20 |
| 21 |
Krouse, Eden (16)
|
LVY |
1:07.37 |
1:10.36 |
2.99 |