| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Margherio, Alexander A (17)
|
MIBBD |
22.54 |
22.52 |
-0.02 |
| 2 |
Phelps, Colton D (17)
|
LERYD |
23.32 |
22.70 |
-0.62 |
| 3 |
Arpasi, Michael J (18)
|
MIBBD |
23.41 |
23.40 |
-0.01 |
| 4 |
Dunlany, Matthew (16)
|
LEYYN |
23.91 |
24.12 |
0.21 |
| 5 |
Puglessi, Ben P (17)
|
MIRAYS |
24.10 |
24.21 |
0.11 |
| 6 |
Bently, John E (16)
|
OHLTPY |
24.82 |
24.24 |
-0.58 |
| 7 |
Schutte, Henry C (16)
|
MIRAYS |
24.32 |
24.27 |
-0.05 |
| 8 |
Losee, Kevin J (16)
|
MIRAYS |
27.32 |
24.35 |
-2.97 |
| 9 |
Diao, Allen X (17)
|
MIBBD |
23.77 |
24.42 |
0.65 |
| 10 |
Parker, Andrew T (17)
|
OHDRY |
25.03 |
24.57 |
-0.46 |
| 11 |
Bottomley, Robert T (15)
|
LERYD |
24.33 |
24.64 |
0.31 |
| 12 |
Lewis, Edward J (16)
|
OHLYST |
24.03 |
24.65 |
0.62 |
| 13 |
Wisler, Alex J (16)
|
OHLTPY |
24.60 |
24.68 |
0.08 |
| 14 |
Tate, Phillip J (17)
|
OHHURY |
24.82 |
24.78 |
-0.04 |
| 15 |
DeLano, Justin T (17)
|
KZY |
24.51 |
24.80 |
0.29 |
| 15 |
Lewis, Michael J (18)
|
OHLYST |
24.56 |
24.80 |
0.24 |
| 17 |
Crowe, Jonathan J (17)
|
MIBBD |
25.23 |
24.85 |
-0.38 |
| 18 |
Boender, Jake F (18)
|
MIRAYS |
28.94 |
24.93 |
-4.01 |
| 18 |
Weidner, Joshua D (18)
|
OHLYST |
25.32 |
24.93 |
-0.39 |
| 20 |
Keane, Spencer I (16)
|
OHEAST |
24.93 |
24.97 |
0.04 |
| 20 |
Hollenbuagh, Ethan (17)
|
OHMFY |
25.27 |
24.97 |
-0.30 |
| 22 |
Betscakos, Maximus G (17)
|
OHMLY |
25.36 |
24.98 |
-0.38 |
| 23 |
Gehring, Andrew G (15)
|
OHLTPY |
24.89 |
25.06 |
0.17 |
| 23 |
Poletta, Daniel L (17)
|
LEGYB |
25.35 |
25.06 |
-0.29 |
| 25 |
Sturgill, Aidan (16)
|
KYBYB |
25.14 |
25.07 |
-0.07 |
| 26 |
LeTourneau, Avery J (15)
|
MIRAYS |
25.51 |
25.09 |
-0.42 |
| 27 |
Orozco, Mitch D (17)
|
OHMCY |
25.34 |
25.12 |
-0.22 |
| 28 |
Heberle, Luke D (15)
|
LERYD |
25.09 |
25.14 |
0.05 |
| 29 |
Lawrence, Jacob H (14)
|
LEYYN |
25.28 |
25.15 |
-0.13 |
| 30 |
Hier, Christopher J (15)
|
MIRAYS |
25.49 |
25.30 |
-0.19 |
| 31 |
Sells, Nick A (15)
|
OHLTPY |
24.93 |
25.32 |
0.39 |
| 31 |
Randle, Nathan D (16)
|
MIBBD |
25.55 |
25.32 |
-0.23 |
| 33 |
Lemire, Matthew (17)
|
MIBBD |
25.11 |
25.47 |
0.36 |
| 34 |
Dafforn Koebler, Griffen (18)
|
INCOLE |
24.84 |
25.54 |
0.70 |
| 35 |
Spees, Xander (14)
|
ZZAMFY |
25.21 |
25.55 |
0.34 |
| 35 |
Villarreal, Ricky (17)
|
RCY |
25.52 |
25.55 |
0.03 |
| 37 |
Johnston, Michael W (18)
|
OHYWSC |
24.65 |
25.59 |
0.94 |
| 38 |
Hendrickson, Andrew (17)
|
OHLYST |
25.54 |
25.62 |
0.08 |
| 39 |
Iversen, Isaac C (15)
|
OHLTPY |
25.08 |
25.72 |
0.64 |
| 40 |
Jones, George K (17)
|
OHHURY |
25.59 |
26.02 |
0.43 |
| 41 |
Schmall, David O (18)
|
MIFFYS |
25.19 |
26.08 |
0.89 |
| 42 |
Brokaw, Robert R (18)
|
OHHURY |
25.26 |
26.22 |
0.96 |
|
Grubbs, Elijah M (15)
|
OHLTPY |
24.91 |
NS |
- |