| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rosenbaum, Anna E (12)
|
LEGO |
1:00.04 |
59.12 |
-0.92 |
| 2 |
Bossart, Lucia (11)
|
OHMLY |
1:03.36 |
1:03.32 |
-0.04 |
| 3 |
Schneider, Katherine L (12)
|
LEGO |
1:04.73 |
1:05.24 |
0.51 |
| 4 |
DeRenzo, Sophia (12)
|
OHLCCY |
1:08.35 |
1:07.54 |
-0.81 |
| 5 |
Francis, Kaitlynn (12)
|
OHLCCY |
1:12.97 |
1:13.65 |
0.68 |
| 6 |
Sines, Abby (11)
|
WAD |
1:15.98 |
1:13.74 |
-2.24 |
| 7 |
Cline, Ariona (12)
|
LCE |
1:16.54 |
1:14.60 |
-1.94 |
| 8 |
Stevens, Paige E (12)
|
LEGYB |
1:15.40 |
1:14.62 |
-0.78 |
| 9 |
Minarcheck, Anastasia (11)
|
OHMLY |
1:17.32 |
1:15.12 |
-2.20 |
| 10 |
Nalepka, Angela (11)
|
OHLCCY |
1:19.35 |
1:18.50 |
-0.85 |
| 11 |
Bemiller, Brynn (11)
|
WAD |
1:16.98 |
1:18.66 |
1.68 |
| 12 |
Restorick, Quinn (12)
|
OHMLY |
1:23.96 |
1:20.76 |
-3.20 |
| 13 |
Siebert, Julia M (11)
|
LEGO |
1:22.90 |
1:22.92 |
0.02 |
| 14 |
Collins, Abigail (11)
|
WAD |
1:29.42 |
1:26.58 |
-2.84 |
| 15 |
Miller, Sadie M (11)
|
LEGYB |
1:31.99 |
1:27.42 |
-4.57 |
| 16 |
Runion, Hazel A (11)
|
LEGO |
1:35.76 |
1:32.17 |
-3.59 |
| 17 |
Thomas, Mia S (11)
|
LEGO |
1:30.23 |
1:34.19 |
3.96 |
| 18 |
Gant, Olivia M (11)
|
LEGYB |
1:35.23 |
1:35.94 |
0.71 |
| 19 |
Raymond, Angel D (12)
|
LEGO |
1:37.28 |
1:39.50 |
2.22 |
| 20 |
Iskra, Evie M (11)
|
LEGO |
1:44.92 |
1:40.08 |
-4.84 |
|
Howard, Madeline C (11)
|
LEGYB |
1:34.80 |
NS |
- |
|
Riedl, Abigail A (11)
|
LEGYB |
1:42.57 |
NS |
- |
|
Kaiser, Kendall (12)
|
OHLCCY |
1:34.08 |
NS |
- |