| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Panizzi, Natalia S (14)
|
AMMOON |
2:22.17 |
2:24.95 |
2.78 |
| 2 |
Nagle, Mia A (14)
|
LEFAST |
2:24.53 |
2:25.38 |
0.85 |
| 3 |
Stockard, Taylor L (14)
|
LERYD |
2:34.46 |
2:29.07 |
-5.39 |
| 4 |
Schrecongost, Alexis G (14)
|
AMUKW |
2:27.03 |
2:30.01 |
2.98 |
| 5 |
Gaguzis, Peri S (14)
|
AMKW |
2:30.99 |
2:30.04 |
-0.95 |
| 6 |
Caniglia, Carrie A (14)
|
LEHEAT |
2:28.68 |
2:32.04 |
3.36 |
| 7 |
Vogel, Morgan P (13)
|
LEMRST |
2:31.43 |
2:32.81 |
1.38 |
| 8 |
Susi, Katie M (14)
|
LEUVFY |
2:27.46 |
2:32.92 |
5.46 |
| 9 |
Reese, Marley K (13)
|
AMMLAC |
2:29.34 |
2:33.60 |
4.26 |
| 10 |
Blakley, Paityn S (13)
|
AMFAST |
2:32.86 |
2:33.67 |
0.81 |
| 11 |
Eiben, Natalie J (13)
|
AMUNJC |
2:34.81 |
2:34.27 |
-0.54 |
| 12 |
MacPhail, Olivia P (14)
|
LEGLSS |
2:33.76 |
2:35.04 |
1.28 |
| 13 |
Patterson, Delaney M (14)
|
AMBVSC |
2:31.71 |
2:36.07 |
4.36 |
| 14 |
Russell, Ella K (14)
|
LELSSC |
2:32.57 |
2:36.42 |
3.85 |
| 15 |
Huang, Isabel S (14)
|
AMPEAQ |
2:37.29 |
2:36.66 |
-0.63 |
| 16 |
Wang, Katalina Y (14)
|
AMPTSC |
2:30.98 |
2:37.19 |
6.21 |
| 17 |
Brooker, Alayna M (13)
|
LEUSTR |
3:03.99 |
2:37.45 |
-26.54 |
| 18 |
Little, Anna R (13)
|
AMNAC |
2:40.32 |
2:37.51 |
-2.81 |
| 19 |
Speelman, Ava M (13)
|
LESTRS |
2:40.66 |
2:38.07 |
-2.59 |
| 20 |
McFaden, Amanda J (13)
|
AMUNJC |
2:39.68 |
2:42.49 |
2.81 |
| 21 |
Reiter, Josephine G (14)
|
AMJCCS |
3:06.38 |
2:42.72 |
-23.66 |
| 22 |
Bonnett, Brenna A (13)
|
AMCSC |
2:44.37 |
2:42.94 |
-1.43 |
| 23 |
Georgagis, Anastasia E (13)
|
AMBVSC |
2:40.15 |
2:43.21 |
3.06 |
| 23 |
Gillespie, Blaire C (13)
|
AMPEAQ |
2:39.72 |
2:43.21 |
3.49 |
| 25 |
Cox, Claire E (13)
|
LECCS |
|
2:43.25 |
- |
| 26 |
Murlin, Darby S (13)
|
LEGLSS |
3:00.58 |
2:43.30 |
-17.28 |
| 27 |
Green, Aubrey M (14)
|
OHFMST |
2:39.39 |
2:43.41 |
4.02 |
| 28 |
McClain, Madelyn G (13)
|
AMNAC |
2:43.60 |
2:43.42 |
-0.18 |
| 29 |
Markowski, Lily M (14)
|
AMPTSC |
2:41.05 |
2:43.71 |
2.66 |
| 30 |
Sharp, Abigail L (14)
|
AMFAST |
|
2:43.82 |
- |
| 31 |
Cunningham, Alexis D (13)
|
LELESD |
2:33.26 |
2:44.19 |
10.93 |
| 32 |
Verbytska, Julia (13)
|
AMMOON |
2:44.08 |
2:44.45 |
0.37 |
| 33 |
Huwar, Sophia G (14)
|
AMANSC |
2:41.02 |
2:45.15 |
4.13 |
| 34 |
Susie, Nicole E (14)
|
AMMOON |
3:07.24 |
2:46.46 |
-20.78 |
| 35 |
Burke, Emma C (13)
|
AMPEAQ |
3:05.37 |
2:49.02 |
-16.35 |
| 36 |
Pophal, Sarah A (13)
|
LELESD |
2:44.00 |
2:49.19 |
5.19 |
| 37 |
Wellman, Evelyn R (13)
|
LELESD |
2:44.49 |
2:51.97 |
7.48 |
| 38 |
Von Ville, Caroline E (14)
|
LEHEAT |
3:06.97 |
2:52.05 |
-14.92 |
| 39 |
Murlin, Elizabeth S (13)
|
LEGLSS |
2:43.89 |
2:52.63 |
8.74 |
| 40 |
Cull, Kennedy M (14)
|
LELESD |
2:44.52 |
2:54.65 |
10.13 |
| 41 |
Smith, Emma C (14)
|
AMMOON |
|
2:57.18 |
- |
| 42 |
Tekieli, Ingrid C (13)
|
LELESD |
2:44.49 |
3:09.83 |
25.34 |