| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lee, Eunji (12)
|
NCGOLD |
35.66 |
33.71 |
-1.95 |
| 2 |
Girouard, Madelyn G (12)
|
NCRMY |
34.04 |
34.09 |
0.05 |
| 3 |
Harper, Gabbrielle E (13)
|
NCGOLD |
35.60 |
34.21 |
-1.39 |
| 4 |
Chambliss, Caroline J (13)
|
NCCFAC |
37.96 |
35.67 |
-2.29 |
| 5 |
Calabrese, Magdalena R (14)
|
NCCFAC |
34.65 |
36.62 |
1.97 |
| 6 |
Becker, Sonya W (12)
|
NCCFAC |
|
36.92 |
- |
| 7 |
Daughtry, Emily P (11)
|
NCGOLD |
35.63 |
38.09 |
2.46 |
| 8 |
Carrano Shepard, Aurora N (12)
|
NCCFAC |
39.96 |
41.50 |
1.54 |
| 9 |
Spencer, Summer R (9)
|
NCCFAC |
46.80 |
46.88 |
0.08 |
| 10 |
Becker, Helena G (10)
|
NCCFAC |
44.04 |
47.90 |
3.86 |
| 11 |
Harris, Violet L (11)
|
NCCFAC |
53.22 |
56.41 |
3.19 |