| # | Swimmer | Team | Seed | Time | Diff |
| 3 |
Mohr, Grace K (16)
|
NCTYDE |
24.81 |
24.99 |
0.18 |
| 5 |
McDevitt, Elizabeth R (17)
|
NCYBAC |
24.94 |
25.40 |
0.46 |
| 6 |
Klosterman, Carstyn J (17)
|
NCTYDE |
24.13 |
25.63 |
1.50 |
| 7 |
Cottingham, Brianna A (16)
|
NCTYDE |
25.59 |
25.66 |
0.07 |
| 9 |
Jennings, Abby C (17)
|
NCTYDE |
25.29 |
25.80 |
0.51 |
| 18 |
Wiles, Morgan E (15)
|
NCTYDE |
25.84 |
26.07 |
0.23 |
| 21 |
McChesney, Shelby K (16)
|
NCTYDE |
25.38 |
26.27 |
0.89 |
| 25 |
Gladden, Carlin R (17)
|
NCTYDE |
26.21 |
26.40 |
0.19 |
| 37 |
Frondoza, Audrianne R (15)
|
NCTYDE |
26.03 |
27.23 |
1.20 |
| 40 |
Kohrt, Lucy E (16)
|
NCTYDE |
26.85 |
27.40 |
0.55 |
| 41 |
Hudson, Katherine J (16)
|
NCTYDE |
26.78 |
27.50 |
0.72 |
| 43 |
Ritter, Rachel E (17)
|
NCYBAC |
27.75 |
27.76 |
0.01 |
| 48 |
Smith, Kelly M (16)
|
NCTYDE |
28.71 |
28.62 |
-0.09 |