| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fore, Daniel G (14)
|
NCTYDE |
24.45 |
24.51 |
0.06 |
| 2 |
Gregory, William D (13)
|
VAWAC |
25.35 |
24.60 |
-0.75 |
| 3 |
Cummings, Nathan L (13)
|
VAWAC |
25.86 |
24.95 |
-0.91 |
| 4 |
Morrow, Connor E (14)
|
NCEAC |
24.56 |
24.97 |
0.41 |
| 5 |
Cayer, Connor N (13)
|
NCEAC |
|
25.01 |
- |
| 6 |
Brooks, Jason D (14)
|
NCGCY |
24.79 |
25.38 |
0.59 |
| 7 |
Woods, Connor E (14)
|
VACCA |
26.43 |
26.35 |
-0.08 |
| 8 |
Votta, Robert J (13)
|
VACCA |
27.22 |
26.82 |
-0.40 |
| 9 |
Allison, Ryan M (14)
|
VAWAC |
27.25 |
27.08 |
-0.17 |
| 10 |
Tonk, Lucas (13)
|
VAWAC |
26.68 |
27.12 |
0.44 |
| 11 |
Maxey, Stone H (13)
|
NCEAC |
25.64 |
27.36 |
1.72 |
| 12 |
Hagadorn, Caleb S (13)
|
VACCA |
26.76 |
27.49 |
0.73 |
| 13 |
Papaioannou, Christos (13)
|
NCTYDE |
28.56 |
27.52 |
-1.04 |
| 14 |
Sianis, Louis E (14)
|
NCEAC |
28.62 |
28.88 |
0.26 |
| 15 |
Thompson, Tyler (13)
|
VACCA |
30.75 |
29.11 |
-1.64 |
| 16 |
Hipps, Tigue J (14)
|
NCGCY |
28.39 |
29.17 |
0.78 |
| 17 |
McDonald, Bryce M (10)
|
NCGCY |
31.37 |
30.44 |
-0.93 |
| 18 |
Hipps, Eamon W (10)
|
NCGCY |
33.86 |
31.73 |
-2.13 |
| 19 |
Ray, Owen M (11)
|
NCGCY |
|
38.62 |
- |
| 20 |
Ray, Lucas J (9)
|
NCGCY |
|
42.50 |
- |
|
Gustafson, Connor T (14)
|
NCUN |
26.73 |
NS |
- |
|
Masneri, Ryan M (10)
|
NCGCY |
32.62 |
NS |
- |
|
Gatling III, Ladaniel (14)
|
NCGCY |
23.46 |
NS |
- |