| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Forst, Preston C (15)
|
NCGCY |
27.86 |
27.72 |
-0.14 |
| 2 |
Nannucci, Pietro (17)
|
NCGCY |
30.09 |
29.86 |
-0.23 |
| 3 |
Esposito, Benjamin R (16)
|
NCGCY |
29.42 |
30.53 |
1.11 |
| 4 |
Muire, Eric N (15)
|
NCGCY |
31.41 |
30.76 |
-0.65 |
| 5 |
Fernandez, Aaron F (16)
|
NCGCY |
31.77 |
31.09 |
-0.68 |
| 6 |
Biles, Bradley W (16)
|
NCGCY |
40.99 |
31.30 |
-9.69 |
| 6 |
Van der Linden, Brandon E (16)
|
NCGCY |
30.23 |
31.30 |
1.07 |
| 8 |
Kornatz, Brendan R (16)
|
NCGCY |
31.64 |
31.86 |
0.22 |
| 9 |
Mills, Brandon R (15)
|
NCGCY |
30.98 |
32.30 |
1.32 |
| 10 |
Carsten, Jace D (16)
|
NCLOY |
36.27 |
32.59 |
-3.68 |
| 11 |
Cox, Bradley D (17)
|
NCFAST |
32.81 |
32.70 |
-0.11 |
| 12 |
McAllister, Ryan D (17)
|
NCLOY |
|
34.02 |
- |
| 13 |
Roussel, Timothee (15)
|
NCLOY |
44.46 |
34.08 |
-10.38 |
|
Herndon, Craig W (16)
|
NCGCY |
30.83 |
NS |
- |