| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ally, Emily H (15)
|
NCGCY |
27.48 |
28.36 |
0.88 |
| 2 |
Pennington, Isabel K (15)
|
NCTYDE |
27.72 |
29.46 |
1.74 |
| 3 |
Campbell, McKenzie N (16)
|
NCGCY |
|
30.29 |
- |
| 4 |
Graham, Anna W (29)
|
NCGCY |
|
30.35 |
- |
| 5 |
Lin, Jennings E (15)
|
NCGCY |
29.35 |
30.56 |
1.21 |
| 6 |
Hoover, Caroline M (16)
|
NCGCY |
47.04 |
30.71 |
-16.33 |
| 7 |
Bunting, Meredith T (16)
|
NCTYDE |
29.32 |
30.77 |
1.45 |
| 8 |
Burton, Piper I (16)
|
NCGCY |
29.81 |
30.86 |
1.05 |
| 9 |
Driscoll, Leanne N (16)
|
NCTYDE |
29.16 |
30.88 |
1.72 |
| 11 |
Thomas, Camden O (15)
|
NCGCY |
30.39 |
31.75 |
1.36 |
| 12 |
Jones, Paige L (17)
|
NCTYDE |
30.23 |
31.95 |
1.72 |
| 13 |
Joyce, Alaina M (18)
|
NCGCY |
29.31 |
32.09 |
2.78 |
| 14 |
Grubb, Sarah E (15)
|
NCGCY |
30.54 |
32.26 |
1.72 |
| 15 |
Randall, Lydia M (15)
|
NCTYDE |
32.65 |
33.72 |
1.07 |
| 16 |
Salthouse, Hayley K (15)
|
NCGCY |
33.08 |
33.99 |
0.91 |
| 18 |
Aldridge, Olivia A (16)
|
NCLOY |
35.23 |
34.66 |
-0.57 |
| 19 |
Sorjonen, Taika A (16)
|
NCGCY |
35.13 |
35.72 |
0.59 |
|
Gilliland, Virginia A (18)
|
NCGCY |
29.92 |
NS |
- |
|
Wolf, Anna K (15)
|
NCTYDE |
30.61 |
NS |
- |