| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bumgarner, Carolina F (12)
|
NCSTAR |
31.85 |
31.37 |
-0.48 |
| 2 |
Shelley, Lilly A (12)
|
NCMHAC |
33.36 |
32.09 |
-1.27 |
| 3 |
Townsend, Abigail C (12)
|
NCSTAR |
34.00 |
32.38 |
-1.62 |
| 4 |
Stevens, Katherine S (12)
|
NCSTAR |
31.85 |
33.09 |
1.24 |
| 5 |
Gribben, Natalie E (11)
|
NCBAQ |
35.70 |
33.98 |
-1.72 |
| 6 |
Auer, Kathleen F (12)
|
NCSTAR |
40.93 |
37.01 |
-3.92 |
| 7 |
Balfour, Genevieve J (11)
|
NCSTAR |
40.82 |
38.02 |
-2.80 |
| 8 |
Rozenbroek, Dirkje J (12)
|
NCBAQ |
42.48 |
38.49 |
-3.99 |
| 9 |
Fontanella, Joslyn R (11)
|
NCSTAR |
47.11 |
40.74 |
-6.37 |
| 10 |
Wallace, Emory E (12)
|
NCSTAR |
44.86 |
47.92 |
3.06 |
| 11 |
Wichman, Charlotte E (11)
|
NCSTAR |
58.32 |
49.12 |
-9.20 |