| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Torres, Nicholas (17)
|
MRBAD |
50.00 |
50.23 |
0.23 |
| 2 |
Harvin, Jackson B (17)
|
GAABSC |
51.80 |
50.32 |
-1.48 |
| 3 |
Borisov, Alexander A (17)
|
SEBAY |
53.55 |
50.56 |
-2.99 |
| 4 |
Octaviano, Antonio T (16)
|
MARAD |
50.51 |
51.08 |
0.57 |
| 5 |
Grespin, Andrew B (17)
|
OHGTAC |
51.00 |
51.20 |
0.20 |
| 6 |
Sungail, Sage Y (14)
|
FLPA |
52.50 |
52.06 |
-0.44 |
| 7 |
Oberle, Jacob A (17)
|
VANOVA |
52.22 |
52.15 |
-0.07 |
| 8 |
Kephart, Wesley L (16)
|
FLSKY |
51.97 |
52.17 |
0.20 |
| 9 |
Edwards, Emerson H (17)
|
SCPALM |
51.99 |
52.18 |
0.19 |
| 10 |
Hensley, Noah C (18)
|
NCTAC |
52.90 |
53.35 |
0.45 |
| 11 |
Taulbee, Jake A (16)
|
GASA |
54.39 |
53.40 |
-0.99 |