| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ssengonzi, Jesse S (16)
|
NCMOR |
56.84 |
59.83 |
2.99 |
| 2 |
Kring, Jaden S (17)
|
NCMOR |
57.15 |
1:00.29 |
3.14 |
| 3 |
Berkowitz, Jeremy M (17)
|
NCYOTA |
58.66 |
1:00.92 |
2.26 |
| 4 |
Boone, Garrett O (15)
|
NCATOM |
58.82 |
1:01.25 |
2.43 |
| 5 |
Meehan, John P (16)
|
NCATOM |
1:00.92 |
1:01.28 |
0.36 |
| 6 |
Smyre, Alexander J (15)
|
NCYOTA |
59.58 |
1:01.68 |
2.10 |
| 7 |
Langston, Mitchell B (18)
|
NCMOR |
58.37 |
1:02.31 |
3.94 |
| 8 |
Tuning, Jeffrey J (17)
|
NCWAVE |
58.67 |
1:03.15 |
4.48 |
| 9 |
Barker, Christopher J (16)
|
NCWAVE |
1:01.11 |
1:05.05 |
3.94 |
| 10 |
Lee, Hyoseon (15)
|
NCWAVE |
1:04.57 |
1:05.63 |
1.06 |
| 11 |
Faber, Samuel S (16)
|
NCYOTA |
59.84 |
1:07.25 |
7.41 |
| 12 |
Smelzer, Peter B (15)
|
NCSGSA |
1:05.14 |
1:09.32 |
4.18 |
| 13 |
Prucha, Jonathan M (14)
|
NCYOTA |
1:01.11 |
1:10.33 |
9.22 |
| 14 |
Lynch, Ryan T (16)
|
NCRSA |
1:11.93 |
1:11.58 |
-0.35 |
|
Adams II, Jeffery A (18)
|
NCATOM |
58.42 |
NS |
- |
|
Rock, Noah L (14)
|
NCGCY |
1:03.98 |
NS |
- |