| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ulrich, Thomas M (17)
|
NCSTAR |
23.25 |
23.27 |
0.02 |
| 2 |
Gardner, Harrison C (15)
|
NCHPSC |
22.87 |
23.45 |
0.58 |
| 3 |
Hall, Spencer M (15)
|
NCHPSC |
24.06 |
23.71 |
-0.35 |
| 4 |
Kuehnert, Samuel A (18)
|
NCXA |
24.82 |
24.13 |
-0.69 |
| 5 |
Hopper, Robert M (17)
|
NCVACB |
23.88 |
24.59 |
0.71 |
| 6 |
Cox, John H (15)
|
NCSTAR |
25.43 |
25.04 |
-0.39 |
| 7 |
MacDermott, Maddux D (16)
|
NCRACY |
25.97 |
25.52 |
-0.45 |
| 8 |
Ballesteros, Daniel A (16)
|
NCHPSC |
25.87 |
25.59 |
-0.28 |
| 8 |
Lennex, Alexander R (16)
|
NCXA |
25.40 |
25.59 |
0.19 |
| 10 |
MacDermott, Connor E (15)
|
NCRACY |
26.79 |
26.41 |
-0.38 |
| 11 |
Murray, Samuel J (16)
|
NCSTAR |
26.09 |
26.65 |
0.56 |
| 12 |
Holloway, Ethan J (16)
|
NCVACB |
26.50 |
27.19 |
0.69 |
| 13 |
Williams, Matheson E (15)
|
NCSTAR |
27.50 |
27.69 |
0.19 |
| 14 |
Lennex, Landon C (15)
|
NCXA |
28.60 |
28.06 |
-0.54 |
|
White, Jackson C (15)
|
NCFISH |
36.96 |
NS |
- |