| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Poelke, Boyd R (16)
|
NCMAC |
52.19 |
54.43 |
2.24 |
| 2 |
Nannucci, Pietro (17)
|
NCSGSA |
53.35 |
55.23 |
1.88 |
| 3 |
Zucker, Colson P (18)
|
NCMAC |
53.42 |
55.71 |
2.29 |
| 4 |
Epler, Robbie J (18)
|
NCMAC |
53.54 |
55.87 |
2.33 |
| 5 |
Walker, John W (16)
|
NCMAC |
50.92 |
56.04 |
5.12 |
| 6 |
Hertweck, Graham E (18)
|
NCSTAR |
54.48 |
56.22 |
1.74 |
| 7 |
Ockert, Jacob A (15)
|
NCRSA |
55.86 |
57.87 |
2.01 |
| 8 |
Atkinson, Isaac K (16)
|
NCMAC |
55.09 |
57.98 |
2.89 |
| 9 |
Heidkamp, George E (16)
|
NCUN |
54.95 |
58.28 |
3.33 |
| 10 |
Rothenberger, Charles A (16)
|
NCMAC |
55.57 |
58.34 |
2.77 |
| 11 |
Watson, William C (15)
|
NCSTAR |
58.71 |
58.45 |
-0.26 |
| 12 |
Barden, Luke H (17)
|
NCSGSA |
55.47 |
58.85 |
3.38 |
| 13 |
Mahoney, Samuel E (18)
|
NCMAC |
58.95 |
58.86 |
-0.09 |
| 14 |
Starbuck, Jason P (15)
|
NCRSA |
59.70 |
58.96 |
-0.74 |
| 15 |
Pritchard, Thomas A (16)
|
NCCAT |
56.18 |
59.50 |
3.32 |
| 16 |
Tuning, Jeffrey J (16)
|
NCWAVE |
49.19 |
59.55 |
10.36 |
| 17 |
Washburn, David W (17)
|
NCRSA |
57.24 |
59.91 |
2.67 |
| 18 |
Montague, Collin R (17)
|
NCCAT |
1:00.15 |
1:00.11 |
-0.04 |
| 19 |
Hering, Flinn M (14)
|
NCSGSA |
1:01.06 |
1:01.07 |
0.01 |
| 20 |
Wachendorfer, Jonathan T (18)
|
NCSTAR |
55.95 |
1:01.66 |
5.71 |
| 21 |
Falise, Nicholas S (15)
|
NCCAT |
55.96 |
1:02.36 |
6.40 |
| 22 |
Dockham, Michael S (17)
|
NCSTAR |
59.17 |
1:02.53 |
3.36 |
| 23 |
Partrick, Michael S (15)
|
NCMOR |
59.51 |
1:03.15 |
3.64 |
| 24 |
Holian, Parker E (14)
|
NCUN |
1:04.60 |
1:03.28 |
-1.32 |
| 25 |
Bria, Eamon A (16)
|
NCCAT |
59.37 |
1:03.31 |
3.94 |
| 26 |
Farabow, Brooks S (15)
|
NCSGSA |
1:03.71 |
1:03.85 |
0.14 |
| 27 |
Saah, Orion J (16)
|
NCMOR |
58.79 |
1:03.88 |
5.09 |
| 28 |
Maycock, Julian L (14)
|
NCSGSA |
1:06.15 |
1:04.80 |
-1.35 |
| 29 |
Sever, Cooper A (13)
|
NCTWA |
1:09.55 |
1:12.41 |
2.86 |